狼疮样BXSB小鼠脾细胞CD134/CD134L的表达及治疗影响

Effect of treatment with Chinese medicine recipe for lupus on the gene expression and protein synthesis of CD134/CD134L in splenic cells of lupus-prone BXSB mice

为了探讨CD134/CD134L共刺激信号在SLE发病机制中的可能作用及治疗的影响。采用狼疮样BXSB小鼠模型,随机分为三组:狼疮方治疗组、强的松治疗组、未治疗组,每组6只,疗程10周。另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组。分别采集上述各组小鼠外周血和脾组织进行检测。结果:(1)与正常对照组比较,未治疗组BXSB小鼠的血清IgG和抗dsDNA抗体水平和脾组织CD4+CD134+、CD8+CD134+、CD19+CD134L+、CD23+CD134L+的百分比及CD134、CD134L的mRNA拷贝数都显著增高(P<0.05或P<0.01)。且血IgG和抗dsDNA抗体水平与脾细胞CD19+CD134L、CD23+CD134L的水平增加呈正相关;(2)狼疮方治疗组或强的松治疗组的血清IgG、抗dsDNA抗体水平和脾组织CD4+CD134+、CD8+CD134+、CD19+CD134L+、CD23+CD134L+的百分比及CD134、CD134L的mRNA拷贝数都显著低于未治疗组(P<0.05或P<0.01),而与正常对照组无显著差异(P均>0.05)。狼疮样BXSB小鼠存在CD134/CD134L的异常表达。狼疮方可减轻狼疮样小鼠高丙种球蛋白血症,减少体内自身抗体产生,对CD134/CD134L的下调作用与强的松相当。

To investigate the effects of treatment with Chinese medicine recipe for lupus on the expression and protein synthesis of CD134/CD134L in splenic cells of lupus prone BXSB mice, 80 male lupus-prone mice used were divided into 3 groups; i.e. untreated group, treated group with lupus recipe and the predisone treated group, and were killed 10 weeks later. Six normal syngeneie C57BL/6 mice were to be used for control. RT-FQ-PCR was used for the detection of the mRNA expres sions of CD134 and CD134L in the splenic cells of mice, and the percentages of the CD4^＋ CD134^＋ , CD8^＋ CD134^＋ , CD19^＋ CD134L^＋ and CD23^＋ CD134^＋ splenic cells were measured by flow cytomelry analysis. Meanwhile, lhe serum levels of total IgG and anti-dsDNA were detected by ELISA. The experimental results showed theft： （1） The serum levels of total lgG and anti dsDNA anllbody in untreated group were higher than those in the other groups, and there was no significant differeces among treated group with lupus recipe, predisone-treated group and control group. （2） The percentages of mRNA expressions on CD4^＋ CD134^＋ , CD8^＋ CD134^＋ , CD19^＋ CD134L^＋ and CD23^＋ CD134L^＋ splenic cells from untreated group of mice were obviously higher than those from the control group. （3） There was a positive correlation between the serum levels of to tal IgG and anti-dsDNA antibody in the untreated group with posttve cell percentages of CD19^＋ CD134L^＋ and CD23^＋ CD134L^＋ cells. （4） In the lupus recipe treated or predisone-treated groups of mice, the positive cell percentages as well as the mRNA expressions of the CD4^＋ CD134^＋ , CD8^＋ CD134^＋ , CD19^＋ CD134L^＋ and CD23^＋ CD134L^＋ cells were .significantly reduced in comparison with the control group, while the difference between the treated and the normal group was not significant. It is concluded that abnormal expression of CD134/CD134L exists in the lupus prone BXSB mice, and the treatment of the Chinese medicine recipe for lupus can reduce the production of IgG and the dsDNA antibody in the lupus-prone mice and the production of autoimmune antibodies in vivo. Its down regulatory effect on CD134/CD1341. is equivalent to thai of predisone.