摘要
以豌豆根瘤菌三叶草生物型(Rhizobium leguminosarum bv.trifolii)TA1和华癸中生根瘤菌(Mesorhizobium huakuii)7653R为供试菌,在测定生长曲线的基础上制备了固体菌剂和浓缩液体菌剂。固体菌剂分别以草炭、蛭石和珍珠岩为载体,比较测定了3种菌剂在室温条件下的保存情况。结果表明在武汉3～9月测定时间内,3种菌剂中以草炭菌剂保存效果最好,成活菌数最多,珍珠岩菌剂次之,蛭石菌剂稍差。同时还比较了保存温度、抑菌剂和保护剂等因素对浓缩液体菌剂根瘤菌存活率的影响,筛选并确定了抑菌剂和保护剂的应用浓度。
Rhizobium leguminosarura bv.trifolii TAI and Mesorhizobiura huakuii 7653R were selected to prepare solid and concentrated inoeulants based on growth curve anlysis. Peat ,vermiculite and pearlite were used to be carriers of solid inoculant. The viability was compared after storage at room temperature from March to September in Wuhan. Results demonstrated that peat was the best, peaflite was the second,and vermiculite was the worst . The effect of storage temperature ,bacteriostatic and protective agents on the viability of concentrated liquid inoculant were compared during storage. Suitable applied concentrations of bacteriostatic and protective agents were selected.
出处
《湖北农业科学》
北大核心
2006年第1期57-61,共5页
Hubei Agricultural Sciences
基金
欧盟RTD国际合作计划(ICA4-CT-2001-10056)资助
国家自然科学基金(30270052)资助
关键词
根瘤菌
生长曲线
固体菌剂
液体菌剂
存活率
rhizobia
growth curve
solid inoculant
liquid inoeulant
viability
