成纤维细胞特异蛋白-1在肺癌中的表达及其临床生物学意义

Expression and clinical biological significance of fibroblast specific protein-1 in human lung cancer tissue

目的研究成纤维细胞特异蛋白-1(S100A4)在人肺癌中的表达及其临床生物学意义,探讨其在肺癌侵袭和转移中的作用。方法采用S-P免疫组化法检测50例肺癌及6例正常肺组织中S100A4的表达水平。结果S100A4在肺癌中表达显著高于正常肺组织;非小细胞肺癌中表达明显高于小细胞肺癌(P<0.05);腺癌中表达明显高于鳞癌(P<0.05)。S100A4表达与非小细胞肺癌临床病理特征关系密切,在有淋巴结转移组与无转移组中两者均差异有统计学意义(P<0.05)。在肺癌不同临床TNM分期中,S100A4阳性表达随临床分期的升高而明显增加,各期间均差异有统计学意义(P<0.05)。S100A4阳性率随肺癌细胞分化程度的降低也有升高趋势,但差异无统计学意义。S100A4表达与肿瘤大小有密切的正相关关系,随肿瘤体积的增大,S100A4的阳性率升高(P<0.05)。结论S100A4与肺癌的侵袭和转移有密切的关系,可以作为评估肺癌病人预后的重要指标。

Objective To observe the expression of fibroblast specific protein- 1 （S100A4） in human lung cancer,and investigate roles of S100A4 in the infiltration and metastasis of lung cancer. Methods immunohistoehemlcal S- P method was used to detect the expression of $100A4 in 50 lung cancer tissues and 6 normal lung tissues. Results The expression of S100A4 was up - regulated in lung cancer tissues. Significant differences of the expression rates of S100A4 was found between lung cancer and normal groups （P 〈 0.05）, between non - small cell lung cancer and small cell lung cancer （P 〈 0.05） and between adenocarcinoma and squamous cell carcinoma （P 〈 0.05 ）. The expression of S100A4 was closely related to elinieopsthological characteristics of non - small cell lung cancer： lymph node metastasis groups had higher S100A4 expressions than without lymph node metastasis groups （P〈0.05）. In TNM stage,the positive expression of S100A4 increased remarkably from Ⅰ to Ⅱ ,then to Ⅲ ＋ Ⅳ （P 〈0.05）. S100A4 had positive relation with tumor volume （P〈0.05） in non-small cell lung cancer. Conclusions S100A4 may play important roles in lung cancer invasion and metastasis, their over expressions could act as a reference to evaluate metastasis and unfavorable prognosis of lung cancer.