植物雌激素白藜芦醇和根皮素对家兔离体主动脉收缩反应的影响(英文)

Effects of phytoestrogens resveratrol and phloretin on contractile response of aortic strips in rabbits

目的探讨植物雌激素白藜芦醇和根皮素对离体主动脉血管收缩的舒张作用特点是否同雌激素以及有关的作用机制。方法将家兔离体主动脉平滑肌条置于灌流肌槽中,记录其等长张力变化。结果白藜芦醇和根皮素可明显抑制离体血管对去甲肾上腺素(NE)、KCl和CaCl2的浓度依赖性收缩反应,使其量效曲线明显右移, pD2′值分别为2.89,3.34, 3.37和3.23, 3.52, 3.77;对KCl预收缩血管条具有浓度依赖性的舒张效应。去除血管内皮、Nω-L-硝基精氨酸(L-NNA)或亚甲蓝对白藜芦醇舒张血管作用具有明显的抑制作用,但对根皮素诱发的血管舒张无明显影响。吲哚美辛和普萘洛尔温育后,对二者的舒血管作用均无明显影响。在无钙Krebs液(含0.01 mmol·L-1EGTA)中,白藜芦醇和根皮素可抑制NE诱发的由肌细胞内钙释放引起的Ⅰ相收缩,但不影响CaCl2诱发的由肌细胞外钙内流引起的Ⅱ相收缩。结论白藜芦醇和根皮素对离体主动脉血管的舒张作用可能与其抑制钙离子内流及细胞内钙释放有关;另外白藜芦醇的舒张作用部分与内皮细胞有关,但根皮素的舒血管作用与内皮细胞无关。

AIM To investigate whether the relaxation characteristics of phytoestrogens resveratrol and phloretin on contractile response of aortic strips are similar to that of estrogen and the mechanisms underground. METHODS Aortic strips from rabbits were suspended in organ baths containing Krebs solution, and then isometric tension was measured. RESULTS Resveratrol and phloretin inhibited the contractile responses to norepinephrine （NE）, KCl and CaCl2, shifted their concentration-response curves rightward with pD2＇ values of 2.89, 3.34, 3.37 for resveratrol and 3.23, 3.52, 3. 77 for phloretin respectively. Also both of them concentration-dependently relaxed KCl-precontracted aortic strip. The relaxing response of resveratrol but not of phloretin in aortic strip was significantly reduced by removal of endothelium or incubation with N^w-L-nitro-arginine and methyhhioninium chloride, however both their relaxant effects were not affected by indometacin and propranolol. In Ca^2＋ -free Krebs solution containing 0.01 mmol· L^-1 EGTA, resveratrol and phloretin inhibited NE-induced contraction which was caused by Ca^2＋ release from intracellular store, but did not affect the contraction which was induced by Ca^2＋ influx. CONCLUSION Resveratrol and phloretin can induce vasorelaxations which may relate to inhibition of Ca^2＋ influx through potential-dependent calcium channels and Ca^2＋ release from intracellular stores, and the relaxing response of resveratrol is endotheliumdependent in part, but of phloretin is not endotheliumdependent.