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VEGF-C及其受体VEGF的表R-3 mRNA在大肠癌达和意义 被引量：5

High expression of VEGF-C and VEGFR-3 mRNA and its implication in colorectal cancer
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摘要 目的研究大肠癌VEGF-C和VEGFR-3 mRNA的表达与大肠癌淋巴管生成的关系。方法采用RT-PCR检测32例新鲜大肠癌及相应正常组织中VEGF-C和VEGFR-3 mRNA表达,免疫组化染色淋巴管内皮特异标记物podoplanin计数淋巴管密度,以血管标记物CD34染色做对比。结果大肠癌VEGF-C及其受体VEGFR-3 mRNA水平显著高于正常组织,且二者表达具有显著正相关性。大肠癌VEGF-C mRNA高表达组癌周淋巴管密度显著高于低表达组。伴有淋巴管受累或淋巴结转移组VEGF-C mRNA显著高于无淋巴管受累或淋巴结转移组。结论VEGF-C/VEGFR-3信号转导途径可能对大肠癌癌周淋巴管生成具有重要作用,并可能进而促进癌组织淋巴结转移。 Objective To study the relationship of expression of VEGFC and VEGFR-3 mRNA with lymphangiogenesis in colorectal cancer. Methods The mRNA expression of VEGF-C and its receptor VEGFR-3 in 32 cases with CRC were detected by RT PCR. The microlymphatic density （MLD） and microvessel density （MVD） were evaluated by immunohistoehemistry with antihuman podoplanin and CDa4 antibody respectively, Results VEGF-C and VEGFR-3 mRNA level of tumor tissues was significantly higher than that in normal mucosa. Moreover, the mRNA expression of VEGFC was significantly coincident and correlated with that of VEGFR-3. VEGF-C mRNA expression was significanlly correlated with MLD at the edge of CRC and with lymphatic involvement and lymph nodes metastasis in CRCs. Conclusion VEGF-C/-D-VEGFR-3 signal pathway might play an important role in peritumoral lymphangiogenesis, and promote lymph node metastasis.
作者 牟江洪 阎晓初 肖华亮 王东 李增鹏 段光杰 向德兵 张沁宏
机构地区 第三军医大学大坪医院野战外科研究所肿瘤中心病理科 第三军医南大学西医院病理学研究所 第三军医大学西南医院病理学研究所
出处 《重庆医学》 CAS CSCD 2006年第3期215-218,共4页 Chongqing medicine
关键词 大肠癌 VEGF-C VEGFR-3 淋巴管生成 转移 colorectal cancer VEGF-C VEGFR- 3 lymphangiogenesis metastasis
分类号 R735.34 [医药卫生—肿瘤] R730.21 [医药卫生—肿瘤]
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参考文献12

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重庆医学
2006年 第3期

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