妇女泌尿生殖道支原体检测和耐药性分析

Detection and drug resistance analysis of mycoplasma from women′s genitourinary tracts

目的了解妇女泌尿生殖道支原体的感染及耐药情况,为临床提供用药参考。方法采用一体化试剂盒,对疑似非淋菌性尿道炎(NGU)女性患者宫颈分泌物进行支原体培养、鉴定及药敏试验。结果441例疑似非淋菌性尿道炎患者中,支原体培养阳性率为35.8%(158例)。其中主要为解脲支原体(Uu)感染,占感染者的81.0%;人型支原体(Mh)阳性率为5.1%;Uu和Mh混合感染阳性率为13.9%。药敏试验显示支原体主要对美满霉素(82.2%)、强力霉素(80.4%)等抗生素敏感,对环丙沙星(50.3%)、螺旋霉素(48.6%)、氧氟沙星(42.7%)等抗生素耐药。结论NGU女性患者支原体感染主要为解脲支原体,对环丙沙星、螺旋霉素、氧氟沙星等抗生素耐药性较高,而对美满霉素、强力霉素等抗生素较敏感,并提示该类抗生素可作为治疗NGU支原体感染的首选药物。

Objective To investigate the infection and drug resistance of mycoplasma from women＇s genitourinary tracts and to provide medication reference to clinic. Methods Mycoplasmas were cultured and identified with using one-step kit, and antibiotic sensitive tests were performed simultaneously. Results Within 441 women suspicius cases of nongonocoeal urethritis（NGU） ,positive rate of mycoplasmas was 35.8% （158 cases）. Among positive cases of mycoplasma, ureaplasma urealytium （Uu） was the major pathogen and its infection rates was 81.0%. Mycoplasma hominis（Mh） was 5. 1% and the mix infection of both Uu and Mh was 13.9 %. In antibiotic sensitive tests, the mycoplasmas were more sensitive to minocycline （82.2% ） and doxycycline （80.4%）, but were more resistive to ciprofloxacin （50.3 % ） and acetylspiramycin （48.6 % ） and ofloxacin （12.7 %）. Conclusion Uu is the major pathogen for infection in NGU of women＇s genitourinary tracts and the minocycline and doxycycline may be drugs of first choice to treat NGU.