不同棉花愈伤组织状态与胚胎发生及其植株再生的关系

Somatic Embryogenesis and Plant Regeneration of Calli Derived from Cotton (Gossypium hirsutumL) Depending on Their Quality

研究结果表明,能够分化出体细胞胚胎并再生成植株的4种愈伤组织状态为:直接产生胚性愈伤组织或体细胞胚胎簇状胚状体、淡黄色且疏松结构胚性愈伤组织、颗粒状疏松结构愈伤组织及褐化、生长缓慢的愈伤组织;难以分化出体细胞胚胎的4种愈伤组织状态为:易发生体细胞胚再愈伤化的愈伤组织、翠绿色表面有硬块状的愈伤组织、易诱导出胚性愈伤组织但不分化成苗的胚性愈伤组织、疯长型愈伤组织。

The results showed that there are four types of calli which own the ability of somatic embryogenesis and regeneration, they are direct somatic embryogenesis from explants, the pale-yellow loose embrygenesis calli directly from explants, granular loose calli, and brown calli with slowly growth; but the other four kinds of calli lose the ability of somatic embryogenesis and plant regeneration, they are easily redifferentiation calli from original calli, green hard surface of calli, calli never inducing embryos, and fast growth calli.