生物气模拟试验的进展

ADVANCES IN SIMULATED TEST OF BIOGAS

“八五”攻关研究在生物气模拟试验方面主要有３个方面的进展：（１）采用了悬浮接种物的方法，即通过弃去一次富集培养物中绝大部分非活性有机质，再经过增殖培养，二次富集提高微生物的浓度和活性，降低了接种物本身的产气量和缩短了试验周斯；（２）将试验温度提高到７５℃，证明了产甲烷菌仍能存活并产甲烷气；（３）对煤和原油进行了模拟试验，证实了煤和原油在厌氧条件下可以降解产气，同时原油厌氧降解也是形成稠油的原因之一，并在稠油中获得了本源产甲烷菌。

Three primary advaces in biogas simulation test of 'eight five-year plan' key tasks are: (1)The method of suspended inocula was used in the simulation. That is to say,by discarding most of the inactive organic matter of the first time cultured micro-organisms, propagated the active ones and cultured them for the second time so as to improve their density and activity. This method could reduce the biogas produced by inocula themselves and shorten test period; (2) When test temperature increased to 75℃, the bacteria could still be alive and produce methane; (3) The simulation test made on coal and crude oil show that, under anaerobic condition, they can be degraded and can produce methane, and this is also one of the reasons of forming dense oil. What's more, dource produced methane bacteria were obtained from crude oil in this simulation test.