与植物多糖硫酸酯结合的HIV-1ⅢB灭活病毒免疫小鼠的研究

Eliciting anti-HIV-1 antibodies with whole-inactivated HIV-1ⅢB bound plant polysaccharide sulfate in mice

目的:为了探讨植物多糖硫酸酯(M33A)与gp120结合后,能否诱导H IV-1ⅢB的gp120暴露出中和抗体的表位,用它作为灭活疫苗以便诱导产生中和抗体。方法:用M33A结合的灭活H IV-1ⅢB作为免疫原,与佐剂混和后,免疫BALB/c小鼠,制备出免疫血浆。用ELISA检测血浆内抗H IV-1特异性IgG抗体的滴度,用改良的活细胞染色法中和试验检测免疫血浆的抗H IV-1ⅢB的中和活性。结果:从与M33A结合的H IV-1ⅢB免疫组的动物获得的免疫血浆内抗H IV-1抗体的滴度(C组:1.5×106;D组:1.5×106)比未结合M33A的H IV-1ⅢB免疫组(4.9×105)高,雌性小鼠的免疫血浆的特异性抗体滴度比雄性的高3倍。所有免疫组获得的免疫血浆均没有抗H IV-1中和活性。结论:M33A与gp120相互作用不能诱导暴露出gp120的中和抗体表位,但M33A可以增强机体免疫原的抗体反应强度,提示它可以作为免疫增强剂用于疫苗研究。

Objectlve：To investigate if a plant-derived polysaceharide sulfate（M33A） binding to HIV-1 gp120 may induce the exposure of neutralization epitopes of gp120, and if M33A-bound HIV-1ⅢB antigens may be used as a AIDS vaccine for inducing neutralizing antibodies against HIV-1. Methods ：Whole-inactivated M33A-bound HIV-1ⅢB antigens were prepared and used to immunize mice after mixing with FCA or FIA. The titers of anti-HIV-1 IgG antibodies in immunized mouse plasma were detected by ELISA,and the HIV-1 neutralization by those plasma was detected by the improved microtiter neutralization assay. Results：M33A-bound HIV-1 antigens induced higher titers of anti-HIV-1 IgG antibodies（group C：1.5×10^6;group D：1.5×10^6） than HIV-1 antigens alone （4.9×10^5 ） ,and female mice produced 3 times higher titers of anti-HiV-1 IgG antibodies than male mice after immunized with various HIV-1 antigens. All three immunization schemes did not induce the production of anti-HIV-1 neutralizing antibodies. Conclusion：M33A binding does not induce gp120 to expose neutralization epitopes. However, M33A may improve the level of mice immune responses to HIV- 1 antigens,suggesting M33A may enhance immune response to HIV-1 antigens.