不同预处理移植后树突状细胞早期重建及与移植物中CD34^+细胞相关性分析

The dendritic cells reconstitution after different allogeneic hematopoietic stem cell transplantation and the correlation with CD34^+ cell dose in allograft

目的:比较不同预处理异基因造血干细胞移植(allo-HSCT)后早期树突状细胞(DCs)亚群重建情况,以及移植物中CD34+细胞是否影响移植后早期DCs亚群重建。方法:采用三色流式细胞仪动态检测不同预处理移植后早期外周血树突状细胞亚群DC1、DC2水平。结果:移植后早期清髓性移植患者体内DCs亚群数量非常低,常规移植组移植后14天与半相合移植组相比,DC1、DC2均无统计学意义(P>0.05)。非清髓性移植组(NST)DC1、DC2高于清髓性移植组,两者相比具有统计学意义(P<0.05)。在30天和60天,所有组DC1、DC2略有波动,但是幅度不大。以输入的CD34+细胞数平均分为三组,三组患者DC1、DC2移植后14、30和60天均无统计学意义(P>0.05)。结论:NST后患者早期DCs重建较清髓性干细胞移植患者早,而常规移植和半相合移植早期DCs重建较慢,二者无差别。移植物中的CD34+细胞不影响移植后早期DCs亚群重建。

Objective：To compare the dendritic cells reeonstitution after different allogeneic hematopoietic stem cell transplantation in early time and if allogeneic graft CD34^ ＋ cell dose correlates with dendritic cells reconstitution after transplantation. Method： Three-colour flow cytometry was applied to study the alteration of the percentage and absolute number in circulating peripheral blood dendritic cells subsets at different time after transplantation. Results：The number of dendritic cell subsets of myeloablative HSCT patients were very low. No difference was observed in the kinetics of DC1 and DC2 reconstitution between normal HSCT group and Haploidentieal HSCT group patients（P 〈0.05）. There was significantly difference between mydoablative HSCT group and normal healthy individuals. There were signliqcant differences between the non-myeloablative HSCT group and the myeloablative HSCT group in the kinetics of DC1 and DC2 reconstitufion（ P 〈 0. 05 ）. We divided the patients into three group by the number of CD34^＋ cell infused. Therefore,we compared the early dendritic cell reconstitution of the three group patients. There was no significantly difference between three group patientsin in different time after transplantation（ P 〈 0. 05 ）. Conclusion ：The early reconstitution of dendritic cells in Nonmyeloablative HSCT patients is earlier than the patients who undergone myeloablative HSCT. Allogeneic graft CD34^＋ cell dose did not correlate with dendritic cells reconstitution after transplantation.