非霍奇金淋巴瘤患者T细胞亚群、NK细胞检测的临床意义

The clinical significance of peripheral blood T cell subsets,NK cells in NHL

目的:研究非霍奇金淋巴瘤(NHL)患者外周血T淋巴细胞亚群、NK细胞检测结果的变化与该病的关系及与慢性淋巴腺炎患者细胞免疫功能的不同变化。方法:采用流式细胞仪(FCM)检测非霍奇金淋巴瘤(NHL)患者、慢性淋巴腺炎及正常人外周血T淋巴细胞亚群比例、NK细胞的变化。结果:非霍奇金淋巴瘤患者与正常人比较总的T淋巴细胞、辅助性T淋巴细胞及CD4+/CD8+比值明显下降(P<0.05),细胞毒性T淋巴细胞明显升高(P<0.05),NK细胞则无明显变化(P>0.05)。非霍奇金淋巴瘤患者与慢性淋巴腺炎患者比较,细胞毒性T淋巴细胞、NK细胞明显升高(P<0.05),而总的T淋巴细胞、辅助性T淋巴细胞无明显改变(P>0.05),CD4+/CD8+比值略有下降但无明显统计学意义。结论:非霍奇金淋巴瘤患者细胞免疫功能明显受到抑制,T细胞亚群及NK细胞的检测对NHL的诊断、治疗、预后判断有一定的临床价值。

Objective：To investigate the relationship of the changes of peripheral bloos T cell subsets and NK cell with nonHodgkin＇s lymphoma（NHL）. And to investigate the change of the cell immune function of NHL and chronic lymphadenitis. Methods： Peripheral blood T cell subsets, NK cell with NHL,chmnic lymphadenitis patients and 50 normal controls were detected quantitatively with FCM. Results： Compared with the normal controls, the number of CD3 ^＋ , CD4 ^＋ T cell, CD4 ^＋/CD8 ^＋ ratio markedly reduced, while those of CD8 ^＋ T cells increased. These were no significant changes in NK cell. Compared with the group of chrnic lymphadenitis, the number of CD8 ^＋ T cell, NK cell markedly increased, while these were no significant changes in CD3 ^＋ , CD4 ^＋ T cell and reduction of CD4.^＋/CD8 ^＋ ratio. Concluslon：NHL patients cell function was suppressed. The detection of T cell subsets and NK cells are clinically valuble on the diagnosis,treatment and prognosis of NHL.