喉癌p53蛋白表达研究

STUDY ON EXPRESSION OFp53 PROTEIN IN LARYNGEAL CARCINOMA

应用抗ｐ５３蛋白革克隆抗体及ＬＳＡＢ免疫织化染色法检测喉癌及声带息肉ｐ５３蛋白的表达。结果显示：２５／４４（５６．９％）喉癌组织呈阳性反应，１６例声带息肉全部呈阴性反应。ｐ５３蛋白表达与喉癌分型、分期及淋巴结转移不相关，而与肿瘤分化程度密切相关（Ｐ＜０．０１）。有吸烟史者ｐ５３蛋白阳性反应数明显高于无吸烟史者（Ｐ＜０．０５）。结果表明：肿瘤抑制基因ｐ５３突变在喉癌发生中可能是一种比较常见的基因改变，与喉癌恶性程度相关，吸烟可能诱发ｐ５３突变。

The monoclonal antibody to p53 and the LtSAB immunohistochemicalmethod were used in this study to detect p53 expression in Laryngeal carcinoma and benignlaryngeal nodules.The results showed that 25(56.9%)of 44 laryngeal carcinomas werepositive but 16 cases of benign lesion were all negative.The differentiation of laryngeal carci-noma was related to expression of p53 protein(P<0.01)and smoking was also related to ex-pression of p53 protein(P<0.05).Our study indicates that overexpression of p53 is not onlyrelated to the laryngeal carcinoma.but also closely related with the malignant level of larynxcarcinoma.Smoking may be a cause of p53 gene mutation.