聚合酶链反应在结核病诊断上的应用研究

STUDAY ON DIAGNOSTIC APPLICATION OF TUBERCULOSIS USING POLYMERASE CHAIN REACTION(PCR)

本文运用ＰＣＲ检测技术，对５５例正常人，３５例非结核性肺部疾病患者，１４６例肺结核病人作对照，用抗酸染色法以及分枝杆菌培养法作对比分析，检测结果为ＰＣＲ检测法阳性率明显高于涂片和培养法，特异性为９７．８％，敏感世为８０％。由于其方法简单，检测速度快，灵敏度高，为结核病的诊断和鉴别诊断提供可靠的依据。

The clinical samples obtained from 55 healthy individuals,35 patients withpulmonory disease of nontuberculosis and 146 patients with pulmonary tuberculosis weremeasured by using techniques of polymerase chain reaction(PCR),acid-fast stain and cultureof mycobacterium.The comparative analyses were detected.The results showed thatusing technique of PCR,the positive rate was significantly height than using techniques ofacid fast stain and culture of mycobacterium.The sensitivity and specificity of PCR for de-tecting M.tuberculosis were 97.8%,80% respectively.The results suggested that techninqueof PCR detecting M.tubeculosis was a useful tool for diagnosis and distinction of pulmonarytuberculosis,since technique of PCR was a rapid,single and significative sensitive method.