改良慢性血清病性大鼠系膜增生性肾炎模型的建立

An improved animal model for mesangioproliferative giomerulonephritis in rat

本文在传统的慢性血清病肾炎模型的基础上,增加了四项改进措施,包括切除大鼠一侧肾脏,用多次足垫皮下注射含牛血清白蛋白(BSA)的福氏佐剂和腹腔内短时注入递增浓度的 BSA 进行预免疫,并采用尾静脉和腹腔注射 BSA 隔日交替进行的免疫方法,同时尾静脉注射大肠杆菌内毒素。缩短了制作周期,致使病变程度相近及病理类型趋向一致。经实验观察及血、尿生化检查和多项病理学检查证实,符合系膜增生性肾小球肾炎的临床和病理特征。

Background Mesangioproliferative glomerulonephritis(MsPGN)is a common pathologic type of glomerulonephritis.A successful animal model of MsPGN is high- ly important for research on glomeru- lonephritis.There are variety of methods to produce of MsPGN in animal model,but some of them are very complicated,time- limited or mild in glomerular lesions.There- for,a MsPGN model in rat has been estab- lisherd by improving method of serum sick- ness nephritis in the present study. Experimental design One-side kidney was removed firstly from the rats.Preimmuniza- tion was performed by repeated injection of BSA and Freund's complete adjuvant into the foot pads.In addition,I.P.injection of inreased concentration of BSA was given for two week,then immunized by interval in- jection of BSA through tail vein and I.P., accompany by the intratailvenal injection of endotoxin of E.coli. Results An abundant hematuria and mild proteinuria had developed in almost all ani- mals in two weeks.Mesangial areas expen- sion and cell proliferation were found in 4 week.Immunofluoresence revealed granular deposits of IgG and C3 in the mesangial area with or without deposition on capilary wall. Mesangial area was filled with large number of the eletron-dense deposits by electron mi- crosopy.The urinary and renal pathologic 1 changes showed here indicate that this method induce a model in rats typical MsPGN. Conclusion Our data indicate that:①re- movel of one-side kidney from rat can ag- gravate the degree of tissue injury in MsPGN model.②endotoxin of E.coli has the function of promoting the development of disease in rat msPGN model.