摘要
目的:探讨冠心康颗粒对大鼠急性心肌缺血的保护作用及其机理。方法:雄性大鼠32只,随机分为4组,每组8只,预防给药7d。灌胃结束后,采用在体大鼠冠状动脉左前降支结扎的方法建立心肌缺血模型,观察冠心康颗粒对大鼠急性心肌缺血后心肌中NO和NOS含量的影响,用免疫组化法检测Bc l-2蛋白在心肌中的表达。结果:模型组、冠心康组、地奥组与假手术组比较,心肌组织NO及NOS含量均有明显降低(P<0.05);而与模型组相比,冠心康组、地奥组的NO及NOS含量均有明显的恢复(P<0.05),提示冠心康颗粒可明显提高大鼠急性心肌缺血后心肌中NO和NOS含量(P<0.005,0.005),同时增加Bc l-2蛋白的表达,减少细胞凋亡。结论:冠心康颗粒对大鼠急性心肌缺血具有保护作用,可能与其提高心肌中NO和NOS含量,增加Bc l-2蛋白的表达有关。
Objective ： To explore the effect and mechanism of Guanxinkang Capsule against myocardium injury induced by acute ischemia in rats. Methods ：32 male rats were randomly divided into 4 groups,8 in every group. After fedding Guanxinkang Capsule for 7 dags preventedly, to ligate forward - descending branch of left coronary artery, establish the injured model and observe the changes of NO and NOS of myocardium. The myocardial expression of Bel -2 was detected by immunohistochemistry. Results ：NO and NOS of myocardium apparentedly decreased in model group,Guanxinkang Capsule group and Diao group（ P 〈 0.05 ）. NO and NOS of Guanxinkang Capsule group and Diao group apparentedly recovery to model group（ P 〈 0.05 ）. This prompted that Guanxinkang Capsule could increase NO and NOS of myocardium in isehemia rats（ P 〈 0. 005,0.005 ） and increase myocardial Bcl -2 protein expression,inhibit myocardial apoptosis. Conclusion ：the effect of Guanxinkang Capsule against myocardium injury induced by acute isehemia in rats may have relationship with increasing NO and NOS of myocardium and expression of Bcl - 2 protein.
出处
《中医药学刊》
CAS
2006年第2期238-241,共4页
Study Journal of Traditional Chinese Medicine
基金
国家科技部新药基金项目(96-901-05-222)
