MPC-BMA共聚物的合成及其血小板粘附行为研究

Study on the Synthesis and Platelet Adhesion Behavior of Phosphorylcholine Copolymers

本研究首先合成了含有磷酸胆碱基团的单体2-甲基丙烯酰氧乙基-2′-三甲胺乙基磷酸酯.内盐(MPC)和甲基丙烯酸正丁酯(BMA)的共聚物,采用红外光谱对其主要基团进行了表征分析,利用血小板粘附实验研究了磷脂聚合物膜的血小板粘附性,通过扫描电镜对血小板在聚合物膜上的形态和粘附量进行观察。结果表明:MPC含量越高,血小板的粘附量和变形程度越小;与其它亲水性单体如HEMA、HPOEM360、HPOEM526相比,等量MPC更能有效的降低其聚合物膜的血小板粘附性。

Phosphorylcholine copolymers were synthesized using monomers of 2-（methacryloyloxy） ethyl-2- （trimethylammonium） ethyl phosphate （MPC） and n-butyl methacrylate （MBA）. The phosphorylcholine groups in copolymers were confirmed by FTIR analysis. The blood compatibility of the phosphorylcholine copolymers was investigated by platelets adhesion and transfiguration using SEM. The results were summarized as following： MPC-BMA eopolymers have better hemocompatibility than Poly （BMA） ; The degree of platelet adhesion and activation decreased with increasing content of MPC in copolymers： Comparing with other hydrophilic acrylate monomers having tethered （poly） ethylene glycol group such as HEMA, HPOEM360 and HPOEM526, MPC deceased platelet adhesion and activation on the copolymer surface more effectively.