不同海拔高度严重烧伤延迟复苏大鼠脑组织中能量负荷的变化及其意义

Changes and significance of cerebral energy charge and cerebral injury in severely burned rats with delayed fluid resuscitation at different altitudes

目的:探讨不同海拔高度严重烧伤延迟复苏大鼠脑组织能量负荷变化及其意义。方法:以120只雄性W istar大鼠建立高原(海拔3 800 m)实验模型(TBSA30%,Ⅲ度),随机分为延迟、即时复苏组和正常对照组,分别于伤后1、6、12、24、72 h及7 d取材。兰州地区取相等数量动物重复实验。应用高效液相色谱法检测脑组织中AMP、ADP、ATP的含量,并计算能量负荷。结果:能量负荷在高原正常对照组与兰州地区正常对照组差异显著(P<0.01)。高原烧伤后脑组织能量负荷与对照组相比均降低,即时复苏组伤后早期即出现显著变化(P<0.01),伤后72 h开始恢复,伤后7 d差异无显著,延迟复苏组伤后7 d仍呈显著差异(P<0.01)。与即时复苏组比较伤后6~24 h无显著差异,伤后72 h^7 d呈显著性差异(P<0.01)。高原地区各时相点与兰州地区比较,EC值均降低,除即时复苏组6 h外,具有统计学意义。结论:不同海拔高度严重烧伤延迟复苏大鼠脑组织能量负荷的改变一定程度上反映了脑损伤的严重程度。

Objective：To explore the relationship between energy charge （EC） and cerebral injury in severely burned rats with delayed fluid resuscitation and its significance at different altitudes. Methods： A total of 120 male Wistar rats were used to establish the experimental models （ TBSA 30% , Ⅲ degree） at high altitude, and they were randomly divided into 3 groups： delayed fluid resuscitation group （HDFR）, immediate fluid resuscitation group （HIFR） and normal control group （HNC）. The samples were harvested at 1,6, 12, 24, 72 h and 7d. The same experiment was repeated in Lanzhou area. The changes of AMP, ADP, ATP and EC in cerebral tissues were detected by high performance liquid chromatography （HPLC）. Results： The level of EC in HNC decreased at every time point compared with LNC （P 〈 0.01 ）. On the early stage post - bum, the significant changes appeared in HIFR （P 〈0.01 ）, and returned to the normal level at 7 d post- burn, but it was still abnormal in HDFR. Compared with each group in Lanzhou area, the content of EC was decreased, and the significant difference appeared at each time point except for 6 h in HIFR （ P 〈 0.01 ）. Conclusion ：The changes of EC might reflect the degree of the pathologic and pathophysiologic changes of cerebral injury in bumed rats at different altitudes.