摘要
目的:探讨乌司他丁(u linastatin,UTI)对缺血再灌注(IR)损伤大鼠肠黏膜免疫屏障的保护作用及机制。方法:采用大鼠小肠缺血45 m in再灌注模型,治疗组于再灌注5 m in内尾静脉缓慢注射UTI。于再灌注后2 h用免疫放射法测定血清、肠黏膜组织中分泌型免疫球蛋白A(sIgA)的含量;四甲基偶氮唑盐微量酶反应比色法(MTT)测定Peyer's淋巴结(pp)、上皮内淋巴细胞(IEL)和固有层淋巴细胞(LPL)增殖活力。结果:肠IR后血清和肠黏膜中sIgA含量下降,淋巴细胞增殖活性下降(P<0.01);不同剂量的UTI治疗组均能逆转IR后sIgA含量下降和淋巴细胞增殖活性下降,差异有显著意义(P<0.01),而不同剂量UTI治疗组间各项指标也有显著差异(P<0.05)。结论:乌司他丁(UTI)可参与调节肠道相关淋巴细胞免疫功能,对缺血再灌注损伤肠道免疫屏障具有保护作用。
Objective： To investigate the effect of ulinastatin on gut mucosal immune function after intestine ischemic reperfusion （IR） injury in rats and its mechanism. Methods： An ischemic reperfusion rat model was used. Ulinastatin was injected into rat＇s caudal vein within 5 min after IR in treatment group. Two hours after IR, the levels of sIgA in plasma and intestinal mucosal were measured by radioimmunoassay. The lymphocytic proliferation activity in gut - associated lymphoid tissue was detected by MTT colorimetric assays. Results： Compared with sham IR group, the level of sIgA in plasma and intestinal mucosal decreased significantly, the lymphocytic proliferation activity in gut - associated lymphoid tissue was depressed remarkably after IR （ P 〈 0.01 ）. Compared with IR group, the level of sIgA in plasma and intestinal mucosal increased and the lymphocytic proliferation activity also increased in low- dose UTI and high -dose UTI groups（ P 〈 0.01 ）. There was a significant difference between low- dose UTI and high -dose UTI groups （P 〈 0.05 ）. Conclusion： Ulinastatin can improve gut mucosal immune function and can protect intestinal mucosal immune barrier from ischemic reperfusion injury.
出处
《西北国防医学杂志》
CAS
2006年第1期48-50,共3页
Medical Journal of National Defending Forces in Northwest China
