人参二醇组皂甙在抗心肌缺血再灌注损伤作用中的钙拮抗剂效应

Calcium antagonist effect of Panaxadiol Saponins on myocardial ischemia and reperfusion injury.

目的研究适宜浓度的人参二醇组皂甙（Parmxadid Sapanins，PDS）在抗心肌缺血再灌注损伤作用中的钙拮抗剂效应。方法大耳白兔30只，体重（2．5±0．3）k；，随机分为对照组与4个实验组，每组6只，制成离体作功兔心模型。St．Thomas心脏停搏液用于对照组，该停搏：液内分别加入浓度为40、80、160、320mg／L的PDS用于4个实验组（以PDS40mg／L组、PDS 80mg／L组、PDS160nag／L组、PDS 320mg／L组表示）。在阻断升主动脉即刻和心脏停搏30min、60min时分别灌注心脏停搏液。于心脏停搏前10min和心脏复搏后心脏作功30min时取心肌标本测定心肌钙离子含量。结果再灌注后PDS 40mg／L组、PDS 80mg／L组、PDS 160mg／L组三个实验组的心肌钙离子含量均明显低于对照组（P〈0．05或P〈0．01），尤以PDS 160mg／L为著。而PDS 320mg／L组的心肌钙离子含量与对照组无显著差异（P〉0．05），甚至有增高倾向。结论在抗心肌缺血再灌注损伤作用中，作为心脏停搏液的添加剂，PDS在40～160mg／L的浓度范围，有钙拮抗剂样作用，且在一定范围内随着浓度递增，该作用增强。但当PDS浓度增至320mg／L时，钙拮抗剂样作用消失。

Objective To study the calcium antagonist effect of Panaxadial Sapanins （PDS） of optimal concentration on myocardial ischemia and reperfusion injury. Methods Thirty isolated working rabbit hearts undergoing myocardial ischemia and reperfusion injury were divided into the control group and four experimental groups randomly, six in each group. St. Thomas＇ solution as the cardioplegic solution were used in the control group, and the PDS 40, 80, 160, 320mg/L as it＇ s additive agent were used in the four experimental gxoups respectively. The cardioplegic solutions were perfused at the moment, 30 minutes and 60 minutes after the aortic cross-clamping respectively. The samples of the myocardial biopsy were taken just in heart working 10 minutes of pre-occlusion and the heart working 30 minutes of heart recovering beat. The calcic content in the myocardium were measured. Results After reperfusion, the calcic content in the myocardium of PDS 40, 80,160mg/L groups were lower than that of control group （P〈0.05 or P〈0.01） and the calcic content of PDS 160rag/L group was the lowest （ P 〈 0.01 ）. There was no significant difference （ P 〉 0.05 ） between the PDS 320mg/L group and the control group of calcic content in the myocardium. Conclusion As the additive agent of the cardioplegic solution, the dosage of PDS in the range of 40- 160mg/L has the calcium antagonist effect on myocardial ischemia and reperfusion injury. And the effect increases when, the concentration of PDS is increased. When the concentration of PDS is increased to 320mg/L, the calcium antagonist effect lost.