冷凝致视网膜色素上皮屏障改变的生物学效应

The biologic effect of cryopexy on retinal pigment epithelium barrier

本文利用动物实验结合光镜电镜及胶体镧示踪对中重度冷凝后不同时间的21只兔眼,84个眼底冷凝灶进行了视网膜色素上皮屏障的研究。结果表明:(1)重度冷凝可引起冷凝区视网膜色素上皮细胞再生不良,冷凝周围区再生的视网膜色素上皮细胞晚期功能失代偿屏障功能不全,脉络膜毛细血管中的胶体镧颗粒进入到视网膜色素上皮细胞内及视网膜下间隙。(2)冷凝对组织的生物学效应与其作用的时间及冷凝头大小有密切关系。(3)中重度冷凝引起的视网膜下间隙的损伤,其分级和Lincoff 及 Kreissig 分级相似,但是这种分级不是绝对的。

With the tracer colloidal lanthanum,light micro- scope and transmission electron microscope in animal experiments,we studied the retinal pigment epithe- lium(RPE)barrier of 48 moderately severe cryoge- nic lesions on the retina of 21 rabbit eyes at dif- ferent intervals after cryopexy.Our findings showed that:1.the repair of disrupted RPE barrier by cryopexy was poor or incomplete,the regenerated RPE cells in restored RPE barrier of some cryogenic lesions showed degeneration and decompensation dur- ing the recovery period,and the tracer in choroidal capillaries leaked into the degenerated RPE cells and the subretinal space;2.the biologic effect of cryo- pexy on the retina was closely related to the acting time and the size of cryoapplication probe;and 3. the damaged grade of subretinal space caused by moderately severe cryopexy was comparable to Lincoff and Kreissig's criteria,but it was not absolute.