摘要
目的探讨胃癌病人外周血中CK20 mRNA的:表达及临床意义。方法采用巢式RT-PCR技术检测70例胃癌病人手术前、后外周血CK20 mRNA的表达情况,并以20例健康志愿者为阴性对照,以胃腺癌细胞株BGC-823作阳性对照。结果CK20 mRNA术前阳性表达率为32.9%,阳性表达率与组织学类型具有相关性(P〈0.01),而与TNM分期、肿瘤的部位、病人性别无明显相关性(P〉0.05)。术后CK20 mRNA阳性表达率明显高干术前(P〈0.05);20例健康志愿者无一例阳性表达,胃腺癌细胞株BCK-823均为阳性表达。结论CK20 mRNA作为标志物用于检测胃癌的血循环微转移是可靠的;手术操作可促使肿瘤细胞释放入血。
AIM To study the clinical significance of the. expression of OK20 mRNA in peripheral blood of gastric cancer. METH. ODS Nested reverse transcription polymerase chain reaction(RT - POR)was employed to test the expression of OK20 mRNA fn BGC - 823 gastric cancer cell line, in peripheral blood of 70 gastric cancer patients pre and post- operatively and 20 healthy donors, RESULTS OK20 mRNA was positive in BGC-823 cell line but in none of healthy donors,in 32.9% of pre- but 62.9% of postoperative patients( P 〈0. 005). CONCLUSION The increase in postoperative OK20 mRNA expression may be caused by surgical maneuver.
出处
《中华腹部疾病杂志》
2006年第2期81-83,共3页
Chinese Journal of Celiopathy
关键词
聚合酶链式反应
胃肿瘤
微转移
细胞角蛋白
Polymerase chain reaction
Gastric neoplasm
Micrometastasis Cytokeratin 20