CK20 mRNA在胃癌病人外周血中的表达及意义

CK20 mRNA expression in peripheral blood of patients with gastric cancer

目的探讨胃癌病人外周血中CK20 mRNA的：表达及临床意义。方法采用巢式RT-PCR技术检测70例胃癌病人手术前、后外周血CK20 mRNA的表达情况，并以20例健康志愿者为阴性对照，以胃腺癌细胞株BGC-823作阳性对照。结果CK20 mRNA术前阳性表达率为32．9％，阳性表达率与组织学类型具有相关性（P〈0．01），而与TNM分期、肿瘤的部位、病人性别无明显相关性（P〉0．05）。术后CK20 mRNA阳性表达率明显高干术前（P〈0．05）；20例健康志愿者无一例阳性表达，胃腺癌细胞株BCK-823均为阳性表达。结论CK20 mRNA作为标志物用于检测胃癌的血循环微转移是可靠的；手术操作可促使肿瘤细胞释放入血。

AIM To study the clinical significance of the. expression of OK20 mRNA in peripheral blood of gastric cancer. METH. ODS Nested reverse transcription polymerase chain reaction（RT - POR）was employed to test the expression of OK20 mRNA fn BGC - 823 gastric cancer cell line, in peripheral blood of 70 gastric cancer patients pre and post- operatively and 20 healthy donors, RESULTS OK20 mRNA was positive in BGC-823 cell line but in none of healthy donors,in 32.9% of pre- but 62.9% of postoperative patients（ P 〈0. 005）. CONCLUSION The increase in postoperative OK20 mRNA expression may be caused by surgical maneuver.