5-FU对人喉癌细胞系Hep-2裸鼠移植瘤治疗作用的实验观察

The Antitumor Effects of 5-FU on Human Laryngeal Squamous Carcinoma Line Hep-2 Transplanted in Nude Mice

目的观察5-氟尿嘧啶对人喉癌裸鼠移植瘤的治疗作用,为临床喉癌的化学药物治疗提供实验依据。方法将10只荷瘤裸鼠随机分为2组,治疗组给予5-氟尿嘧啶30mg/kg腹腔注射;对照组给予生理盐水0.2ml腹腔注射,连续给药7天后改为隔日给药,治疗后30天实验结束。观察移植瘤的生长特性和裸鼠体重增长值及移植瘤的光镜和电镜切片。结果与对照组相比,治疗组肿瘤体积增长较对照组缓慢,瘤体积和重量均明显低于对照组,统计学上有显著性差异(P<0.05),表明5-FU可有效抑制人喉癌裸鼠移植瘤的生长。治疗前后裸鼠体重增长值差别无显著性意义(P>0.05)。光镜和电镜观察治疗组移植瘤主要呈坏死改变。结论5-氟尿嘧啶可有效抑制人喉癌细胞系Hep-2裸鼠移植瘤的生长,体积抑瘤率为60.70%,重量抑瘤率达65.95%。抗肿瘤作用的主要形态学改变主要是药物直接作用于肿瘤细胞导致细胞的坏死。

Objective To determine the effects of 5-FU on the tumor growth of human laryngeal carcinoma llne Hep-2 transplanted in nude mice and guide the clinical chemotherapy in laryngeal carcinoma. Methods The 10 mice bearing substaneous transplanted tumour were divided into two groups randomly. The treatedgroup were given 5-FU （30mg/kg） by abdominal administration for consecutively 7 days, then once every other day. The mice＇s condition and the character of tumour growth were evaluated. The his-topathological studies with light and transmission microscopy was also done. Results The growth of the carcinoma implanted in the nude mice were significantly inhibited in the treated group. Compared with the control group, the implanted tumours were significantly reduced in weight and volume in mice treated with 5-FU （P〈0.05 ） There was no significantly difference in weight increasing between the two group （P 〉0.05） . The main morphological character of the treated tumour is nerosis. Conclusions 5-FU could suppress the tumour growth and the growth inhibition rate could reach 60.70% （ turnout volume） and 65.95% （tumour weight） Inducing tumour cell nerosis may be the main morphological change of 5-FU＇s antitumour mechanism in larygeal carcinoma.