葛根素对胰岛素抵抗大鼠脂肪组织中糖原合成酶激酶3β表达的影响

Effect of puerarin on glucogen synthase Kinase-3 bate in adipocyte of rats with insulin resistance

目的探讨葛根素对高脂饮食诱导的胰岛素抵抗大鼠脂肪组织中糖原合成酶激酶3β(GSK-3β)表达的影响。方法30只SPF级雄性Wister大鼠随机分为2组,正常对照组10只,常规饲料喂养,模型组20只,高脂饲料喂养;4周后模型形成,模型组随机分为2组(胰岛素抵抗组、葛根素干预组,每组10只),继以高脂饲料喂养,葛根素干预组同时给予葛根素注射液100mg·kg-1·d-1腹腔注射。干预6周后,用Western-blot方法检测各组大鼠附睾脂肪组织中GSK-3β的表达,分析对比各组的表达差异;定期检测大鼠体重、空腹血糖及血浆胰岛素、血甘油三酯和胆固醇,并计算胰岛素敏感指数。结果高脂饲料喂养4周后,与正常对照组比较,模型组大鼠体重(BW)、空腹血糖(FPG)、胰岛素(FINS)、胆固醇(TC)、甘油三酯(TG)显著升高(P<0.05或P<0.01),胰岛素敏感指数ISI显著下降(P<0.01),胰岛素抵抗模型诱导成功;葛根素干预6周后,与胰岛素抵抗组大鼠比较,脂肪组织中GSK-3β的表达显著下降(P<0.05),和正常对照组比较,无显著差异。结论葛根素显著下调脂肪组织GSK-3β的表达,并且可能参与其改善胰岛素抵抗的机制。

AIM： To investigate the effect of puerarin on expression of the glucogen synthase kinase-3 bate （GSK-3β） in adipocyte of rats with insulin resistance induced by diets. METHODS： 30 Wister rats were randomized into two groups： control group （n = 10） and model group （ n = 20）. Control group were fed with general diets, while model group were fed with the high fat diets to induce insulin resistance. After 4 weeks, the models have formed then were randomized into two groups： model group （ n = 10） and puerarin group （ n = 10）. Puerarin group were treated with puerarin （ 100 mg·kg^-1·d^-1, ip） for 6 weeks. GSK-3β protein was detected by Western-blot analysis. Body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected termly and the insulin sensitivity index was calculated. RESULTS： The model group showed hyperglycemia, hyperinsulinism, obviously obesity and the insulin resistance index （ISI） decrease by high fat diets, insulin resistance was induced by this way. Compared with model group, GSK-3β protein in adipocyte of puerarin group rats decreased （P 〈 0.05 ）. CONCLUSION： Puerarin can decrease the expression of GSK-3β protein in adipocyte of rots with insulin resistance which may be involved in the mechanism of amelioration the state of insulin resistance.