摘要
目的:研究慢性高眼压动物模型的制作方法。方法:选择20只健康Wistar大鼠,雌雄不限,随机分为高眼压组(10只)和sham对照(假手术)组(10只)。高眼压组采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流升高眼压,而sham对照组只剪开球结膜,未进行巩膜表面静脉以及角膜缘周围血管电凝。分别干术后1,2,3,4及8WK测大鼠双眼压,结果进行统计学分析。结果:高眼压组右眼术后眼压明显升高。术后1 Wk眼压:(30.35±4.98)mmHg(1 kPa=7.5mmHg),术后1-8wk间眼压基本稳定。术后各时间点高眼压组右眼眼压与术前、左眼及sham对照组右、左眼间比较。差异有统计学意义(P<0.001)。结论:采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流能成功复制慢性高眼压动物模型。
AIM: To study the experimental method of inducing the chronic intraocular hypertension in rat eyes.
METHODS: Twenty Wistar rats were randomly divided into ocular hypertension and Sham control groups. Intraocular pressure (IOP) was raised by electrocoagulating at least 3 episcleral and limbal veins on the right eye of each rat in ocular hypertension group and its contralateral eye was used as control. At 1, 2, 3, 4 and 8wk after the electrocoagulation of the veins, IOP were measured.
RESULTS: The treatment of electrocoagulation caused a significant IOP increase of the right eyes over the baseline, over the contralateral eyes, and over the sham control eyes (repeated measures ANOVA, P〈0.001). At lwk, IOP was (30.12±5.18 ) mmHg (1kPa=7.SmmHg), and maintained the high IOP up to 8wk.
CONCLUSION: The chronic intraocular hypertension model could be successfully created by electrocoagulating three or more episcleral and limbal veins.
出处
《国际眼科杂志》
CAS
2005年第6期1090-1092,共3页
International Eye Science
基金
中国四川省教育厅自然基金资助(No.2005A074)~~
关键词
青光眼
高眼压
动物模型
glaucoma
intraocular hypertension
animal model