制作慢性高眼压动物模型的一种新方法——水下电凝巩膜表面静脉法(英文)

A new method of duplication of the chronic intraocular hypertension model: underwater electrocoagulation

目的：研究慢性高眼压动物模型的制作方法。方法：选择20只健康Wistar大鼠,雌雄不限,随机分为高眼压组(10只)和sham对照(假手术)组(10只)。高眼压组采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流升高眼压,而sham对照组只剪开球结膜,未进行巩膜表面静脉以及角膜缘周围血管电凝。分别干术后1,2,3,4及8WK测大鼠双眼压,结果进行统计学分析。结果：高眼压组右眼术后眼压明显升高。术后1 Wk眼压：(30．35±4．98)mmHg(1 kPa=7．5mmHg),术后1-8wk间眼压基本稳定。术后各时间点高眼压组右眼眼压与术前、左眼及sham对照组右、左眼间比较。差异有统计学意义(P<0．001)。结论：采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流能成功复制慢性高眼压动物模型。

AIM： To study the experimental method of inducing the chronic intraocular hypertension in rat eyes. METHODS： Twenty Wistar rats were randomly divided into ocular hypertension and Sham control groups. Intraocular pressure （IOP） was raised by electrocoagulating at least 3 episcleral and limbal veins on the right eye of each rat in ocular hypertension group and its contralateral eye was used as control. At 1, 2, 3, 4 and 8wk after the electrocoagulation of the veins, IOP were measured. RESULTS： The treatment of electrocoagulation caused a significant IOP increase of the right eyes over the baseline, over the contralateral eyes, and over the sham control eyes （repeated measures ANOVA, P〈0.001）. At lwk, IOP was （30.12±5.18 ） mmHg （1kPa=7.SmmHg）, and maintained the high IOP up to 8wk. CONCLUSION： The chronic intraocular hypertension model could be successfully created by electrocoagulating three or more episcleral and limbal veins.