HMGN2的重组表达质粒的构建及其抗菌功能研究

Construction of recombinant prokaryotic expressing plasmid of HMGN2 and study on its antimicrobial activity

目的:构建HMGN2重组表达载体,探讨HMGN2分子抗菌作用。方法:用基因克隆的方法构建pET-32 a-c(+)-HMGN2重组表达质粒,经IPTG诱导表达后,用亲和层析的方法纯化H is-HMGN2融合蛋白,Tric ine-SDS-PAGE电泳鉴定其分子量,行琼脂糖弥散法检测其抗菌功能。结果:成功构建了pET-32 a-c(+)-HMG17重组表达质粒,其表达的融合蛋白对致病性大肠杆菌54080,54299以及金黄色葡萄球菌ATCC25923均有抗菌活性。结论:HMGN2是一种抗菌分子,可能参与了体内的天然免疫防御作用。

Objective： To express and purify recombinant HMGN2 protein and investigate its antimicrobial activity. Methods： The recombinant prokaryotic expressing plasmid pET-32a-c （ ＋ ）-HMGN2 was constructed by gene cloning. Induced by IPTG, the fused protein with the histidine-tagged was purified by HisTrap Kit and identified by Tricine-SDS-PAGE. The antimicrobial activity was detected by agar diffusion test. Results： The recombinant HMGN2 protein was against the pathogenic strains E. coli 54080,54299 and S. aureus. ATCC 25923. Conclusion： HMGN2 protein is a antimicrobial molecule and it is probably involved in the defensive of the innate immunity in vivo.