AM真菌孢子不同消毒方法的优化研究

ON OPTIMIZATION OF DIFFERENT SRERILIZATION METHODS FOR ARBUSCULAR MYCORRHIZAL FUNGUS SPORES

采用7种消毒方法,对Gigaspora margarita,Glomus mosseae和Glomus versiforme3种丛枝菌根真菌孢子分别进行表面消毒试验.结果表明,Gigaspora margarita的孢子,采用5%氯胺T+0.04%链霉素消毒液+2滴Tween 20处理15min,无菌蒸馏水漂洗6～10次的消毒方法最好,萌发率高,且消毒效果好;Glomus mosseae和Glomus versiforme2种孢子,可采用上述方法或'差别灭菌法',即先用2%氯胺T+200 mg/L链霉素+100 mg/L庆大霉素消毒液+2滴Tween20处理10 min,无菌蒸馏水漂洗6～10次后,置于26℃条件下培养3 d,再用2%氯胺T+200 mg/L链霉素+100 mg/L庆大霉素消毒液+2滴Tween 20处理10 min,最后用无菌蒸馏水漂洗6～10次即可.这2种方法都非常好,消毒效果没有显著差异.

Seven methods were designed for spore surface sterilization of Gigaspora margarita, Glomus mosseae and G. versiforme. Sarisfar＇tory sterilization was achieved for the spores of G. margarita, when they were treated for 3 min in a solution of 5% chloramine T ＋ 0.04% streptomysin ＋ 2 drips of Tween 20 and then washed 6 - 10 times with aseptic distilled water. For the spores of G. mosseae and G. versiforme, the above method gave satisfactory results and another method, the differential methods of sterilization＂ was also effective, i. e. to sterilize the spores for 10 min in a solution of 2% chloramine T ＋ 200 mg/L streptomycin ＋ 100 mg/L gentanlicin ＋ 2 drips of. Tween 20 and then wash them 6 - 10 times with aseptic distilled water. After that, the spores were made to germinate for 3 days at 26℃ and then sterilized for 10 min in a solution of 2% chloramine T ＋ 200 mg/L streptomycin ＋ 100 mg/L gentamicin ＋ 2 drips of Tween 20 and then wash them 6 - 10 times with aseptic distilled water. Both the 2 procedures worked very well and no significant difference was found hetween them in their effectiveness.