Morphological and serum hyaluronic acid, laminin and type Ⅳ collagen changes in dimethylnitrosamine-induced hepatic fibrosis of rats

瞄准：学习词法并且浆液透明质酸(哈) ， laminin (行) ，和类型 IV 骨胶原在 dimethylnitrosamine (DMN ) 导致的老鼠的肝的纤维变性变化。方法：肝纤维变性的老鼠模特儿被 DMN 劝诱。浆液哈，类型 IV 骨胶原，和行被 ELISA 测量。肝 / 重量索引和词法变化在 d 上在电子显微镜下面被检验 7， 14， 21，和 28 由免疫组织化学的高山哈象天狼星红一样染色的平滑肌肌动朊 alpha-SMA 并且他染色。结果：浆液的层次哈，类型 IV 骨胶原和行显著地从 d 增加了 7 到 d 28 (P = 0.043 ) 。肝 / 重量索引在 d 上增加了 7 并且在 d 上减少了 28。在模型组，老鼠肝染色了与他和天狼星红在中央静脉显示出明显的出血和坏死肝 d 上的 10 腹片 7。薄纤维变性氏族一被形成在 d 上加入肝的中央区域 14。alpha-SMA 的数字积极房间显著地在模型组被增加。过渡肝的星形细胞在电子显微镜下面被观察。在模型组的所有老鼠在肝的实质和 alpha-SMA 的一个网络显示出微榴状的纤维变性积极房间。典型 myofibroblasts 在含纤维的中隔的核心被嵌入。比作控制组，骨胶原纤维变性的区域密度百分比并且病理学的分级在不同 d (7， 14，和 28 ) 上在模型组(P【0.05 ) 是显著地不同的。在肝的织物的骨胶原纤维变性的区域密度百分比与浆液的层次有积极关联哈，行，和类型 IV 骨胶原。结论：词法并且浆液哈，类型 IV 骨胶原，和行在老鼠在导致 DMN 的肝纤维变性被改变。

AIM： To study the morphological and serum hyaluronic acid （HA）, laminin （LN）, and type IV collagen changes in hepatic fibrosis of rats induced by dimethylnitrosamine （DMN）.METHODS： The rat model of liver fibrosis was induced by DMN. Serum HA, type IV collagen, and LN were measured by ELISA. The liver/weight index and morphological changes were examined under electron microscope on d 7, 14, 21, and 28 by immunohistochemical alpha smooth muscle actin α-SMA staining as well as Sirius-red and HE staining.RESULTS： The levels of serum HA, type IV collagen and LN significantly increased from d 7 to d 28 （P = 0.043）. The liver/weight index increased on d 7 and decreased on d 28. In the model group, the rat liver stained with lie and Sirius-red showed evident hemorrhage and necrosis in the central vein of hepatic 10 Iobules on d 7. Thin fibrotic septa were formed joining central areas of the liver on d 14. The number of α-SMA positive cells was markedly increased in the model group. Transitional hepatic stellate cells were observed under electron microscope. All rats in the model group showed micronodular fibrosis in the hepatic parenchyma and a network of α-SMA positive cells. Typical myofibroblasts were embedded in the core of a fibrous septum. Compared to the control group, the area-density percentage of collagen fibrosis and pathologic grading were significantly different in the model group （P〈0.05） on different d （7, 14, and 28）. The area-density percentage of collagen fibrosis in hepatic tissue had a positive correlation with the levels of serum HA, LN, and type IV collagen.CONCLUSION： The morphological and serum HA, type IV collagen, and LN are changed in DMN-induced liver fibrosis in rats.