Expression and immunoreactivity of HCV/HBV epitopes

AIM： To develop the epitope-based vaccines to prevent Hepatitis C virus （HCV）/Hepatitis B virus （HBV） infections. METHODS： The HCV core epitopes C1 STNPKPQRKTKRNTNRRPQD （residuals aa2-21） and C2 VKFPGGGQIVGGVYLLPRR （residuals aa22-40）, envelope epitope E GHRMAWDMMMNWSP （residuals aa315-328） and HBsAg epitope S CTTPAQGNSMFPSCCCTKPTDGNC （residuals aa124-147） were displayed in five different sites of the flock house virus capsid protein as a vector, and expressed in E. coli cells （pET-3 system）. Immunoreactivity of the epitopes with anti-HCV and anti-HBV antibodies in the serum from hepatitis C and hepatitis B patients were determined. RESULTS： The expressed chimedc protein carrying the HCV epitopes C1, C2, E （two times）, L3C1-I2E-L1C2- L2E could react with anti-HCV antibodies. The expressed chimeric protein carrying the HBV epitopes S, I3S could react with anti-HBs antibodies. The expressed chimeric proteins carrying the HCV epitopes C1, C2, E plus HBV epitope S, L3C1-I2E-L1C2-L2E-I3S could react with anti- HCV and anti-HBs antibodies. CONCLUSION： These epitopes have highly specific and sensitive immunoreaction and are useful in the development of epitope-based vaccines.

AIM: To develop the epitope-based vaccines to prevent Hepatitis C virus (HCV)/Hepatitis B virus (HBV) infections.METHODS: The HCV core epitopes C1 STNPKPQRKTKRNTNRRPQD (residuals aa2-21) and C2 VKFPGGGQIVGGVYLLPRR (residuals aa22-40), envelope epitope E GHRMAWDMMMNWSP (residuals aa315-328) and HBsAg epitope S CTTPAQGNSMFPSCCCTKPTDGNC (residuals aa124-147) were displayed in five different sites of the flock house virus capsid protein as a vector, and expressed in E. coli cells (pET-3 system).Immunoreactivity of the epitopes with anti-HCV and anti-HBV antibodies in the serum from hepatitis C and hepatitis B patients were determined.RESULTS: The expressed chimeric protein carrying the HCV epitopes C1, C2, E (two times), L3C1-I2E-L1C2-L2E could react with anti-HCV antibodies. The expressed chimeric protein carrying the HBV epitopes S, I3S could react with anti-HBs antibodies. The expressed chimeric proteins carrying the HCV epitopes C1, C2, E plus HBV epitope S, L3C1-I2E-L1C2-L2E-I3S could react with antiHCV and anti-HBs antibodies.CONCLUSION: These epitopes have highly specific and sensitive immunoreaction and are useful in the development of epitope-based vaccines.