Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-1_(19)

AIM： To construct the recombinant Lactococcus/actis as oral delivery vaccination against malaria. METHODS： The C-terminal 19-ku fragments of MSP1 （MSP-119） of Plasmodium yoelii265-BY was expressed in L. lactis and the recombinant L. lact/s was administered orally to BALB/c and C57BL/6 mice. After seven interval vaccinations within 4 wk, the mice were challenged with P. yoelii 265-BY parasites of erythroo/tic stage. The protective efficacy of recombinant L. lactiswas evaluated. RESULTS： The peak parasitemias in average for the experiment groups of BALB/c and C57BL/6 mice were 0.8± 0.4% and 20.8±26.5%, respectively, and those of their control groups were 12.0±0.8% and 60.8±9.6%, respectively. None of the BALB/c mice in both experimental group and control group died during the experiment. However, all the C57BL/6 mice in the control group died within 23 d and all the vaccinated mice survived well. CONCLUSION： The results imply the potential of recombinant L. lactis as oral delivery vaccination against malaria.

瞄准：构造 recombinant Lactococcus 乳酸作为对疟疾的口头的交货种痘。方法：变形体 yoelii 265-BY 的 MSP1 (MSP-1 (19 )) 的 C 终端 19-ku 碎片在 L 被表示。乳酸是并且 recombinant L。乳酸是予 BALB/c 和 C57BL/6 老鼠口头上地被以。在在 4 wk 以内的七间隔种痘以后，老鼠与 P 被质问。红细胞舞台的 yoelii 265-BY 寄生虫。recombinant L 的保护的功效。乳酸被评估。结果：分别地，在为 BALB/c 和 C57BL/6 鼠标的实验组的一般水准的山峰 parasitemias 是 0.8+/-0.4% 和 20.8+/-26.5% ，他们的控制组的那些分别地是 12.0+/-0.8% 和 60.8+/-9.6% 。任何一个都没在实验期间死于在试验性的组和控制组的 BALB/c 老鼠。然而，在控制组的所有 C57BL/6 鼠标在 23 d 和熬过很好的所有种牛痘的鼠标以内死了。结论：结果暗示 recombinant L 的潜力。乳酸作为对疟疾的口头的交货种痘。