皂化法分离测定三孢布拉氏霉菌体中辅酶Q_(10)的研究

Extracting and Determining Coenzyme Q_(10) from Blakesleatrispora by Saponification

目的：为研究醇碱皂化法分离三孢布拉氏霉菌体中辅酶Q10的最佳工艺。方法：采用正交实验，对醇碱皂化法分离测定三孢布拉氏霉菌体中辅酶Q10的各工艺条件进行了研究。结果：皂化时间、醇碱浓度及焦性没食子酸添加量对皂化效果影响显著，而温度（50—90℃）对其影响较小。醇碱皂化法提取三孢布拉氏霉中辅酶Q10的最佳工艺条件为：KOH加入量为湿菌体的1／2（w／w），醇碱浓度为10％，焦性没食子酸量添加量为湿菌体的10％（w／w），在70℃下皂化60min。此提取工艺平均回收率达81．8％，RSD为2．6％。

Objective：The extracting and determining of Co Q10 from Blakeslea trispora using saponifieation was studied to get the best craft condition .Methods：The extracting and determining of CoQ10 from Blakeslea trispora using saponifieation was studied by orthogonal test. Results：It was proved that saponified time, alkali concentration and the added pyrogallol affected the saponification greatly, while the temperature（ from 50℃ to 90℃）had less effect. The optimum extracting condition were as the following： Wet culture was distilled at 70℃ for 60min, added caustic potash up to 1/2 wet culture（w/w）, pyrogallol up to 10% wet culture（w/w）, and the concentration of alkali - methanol was 10% .The average recovery of CoQ10 was 81.8% ,with RSD of 1.34%.