GAL4/UAS系统在转基因技术中的应用研究进展

Study of GAL4/UAS System in Transgenic Technology

GAL4/UAS系统是一种转基因技术体系,其原理是利用特定的启动子或增强子,以组织特异性的方式激活酵母转录激活子GAL4的表达,GAL4又以同样的方式引起GAL4反应元件(UAS)-靶基因的转录。GAL4/UAS系统的关键点在于:GAL4基因和UAS-靶基因分别存在于两个转基因系中。GAL4转基因系中有转录激活子,但没有靶基因;在UAS-靶基因系中,转录激活子不存在,因而靶基因处于沉默状态,只有将GAL4转基因系与UAS-靶基因系进行杂交,才可能产生表达靶基因的后代。本文综述了GAL4/UAS系统的建立及其研究应用。

GAL4/UAS system is a transgenic system. In this system, a special promoter is used to direct expression of the yeast transcriptional acti- vator GAL4 in a particular pattern, and GAlA in turn directs transcription of the GAL4- responsive （UAS） target gene in an identical pattern. The key feature of the GAlA system is that the GAlA gene and UAS - target gene are initially separated into two distinct transgenie lines. In the GAL4 line, the activator protein is present, but has no target gene to activatc. In the UAS line, the target gene is silent in the absence of activator. When the GAlA and UAS lines are crossed, the target gene is turned on only in the progeny of the cross. It was reviewed here the study and the aPPlication of the GAL4/UAS system at present.