β-防御素-2在不同月龄大鼠肺组织表达研究

Study on the expression of β-defensin-2 gene in the pulmonary tissues of rats of different months

目的研宄老龄（18-20月）和青年（2～3月）大鼠肺组织表达β-防御素-2（β-defensin-2，BD-2）的量有无差别。方法老年和青年健康Wistar大鼠各10只，采用逆转录一多聚酶链反应方法检测肺组织BD-2基因表达水平；HE染色切片来了解肺组织的情况。结果老龄大鼠肺组织BD-2基因表达水平为0．208±0．138，青年为0．351±0．206，2组差别无显著性（P〉0．05）。HE染色切片老年大鼠肺组织可见肺泡断裂、肺间隔增厚、酸性细胞渗出等改变，而青年大鼠没有上述改变。结论基因水平上青年大鼠肺组织BD-2稍高于老龄大鼠，但无统计学意义，提示老年人易患呼吸道感染与BD-2的表达可能无关，但仍需进一步的研究。

Objective To investigate the exspression of β-defcnsin-2 （BD-2） gene in the pulmonary tissues of young （2-3 months） and old （ 18-20 months） rats, Methods Total RNA was isolated from the pulmonary tissues of the rats. BD-2 mRNA was measured by RT-PCR. The pulmonary tissues were observed by H＆E dyeing. Results The expression of BD-2 gene was lower in the old rats（0. 208 ±0. 138） than that （0. 351±0. 206） in the young rats,but there was no statistical difference. The microscope showed that in the elderly,there were destruction of alveolar tissue, pulmonary interstitium thickness and so on. Conclusions There was no statistical difference in the expression of BD-2 gene between the young and the old rats,which suggested that there may be no correlation between β-defensin-2 gene and the susceptibility to all kinds of microorganism in the elderly.