鹅膏毒肽致肝脏损伤的生化指标探讨

Research on the biochemical index for liver damage induced by amatoxin

目的:探讨敏感的鹅膏毒肽致肝脏损伤的早期临床诊断生化指标。方法:选SD大鼠24只,随机分为4个组(对照组和鹅膏毒肽低、中、高剂量组)。根据动物试验的LD500.3～0.6m g/kg染毒组剂量依次为0.1、0.3、0.5m g/kg,染毒后测定血清羟脯氨酸、氨基己糖含量。结果:中、高剂量染毒组大鼠血清羟脯氨酸、氨基己糖含量显著低于对照组。结论:血清羟脯氨酸、氨基己糖含量可以反映鹅膏毒肽对肝细胞的损伤情况,其可能是鹅膏毒肽中毒早期临床诊断的敏感生化指标。

Objective To explore the biochemical indexes for hepatic cell damage induced by amatoxin in the early clinical diagnosis. Methods 24 rats were randomly divided into four groups： control group, low-dose with 0. 1 mg/kg group, median-dose with 0. 3 mg/kg group, high-dose with 0.5 mg/kg group （LD50 0.3-0.6 mg/kg） . The levels of hydroxide proline and hexosamine were detected after administration of amatoxin. Result The levels of hydroxide proline and hexosmaine in administration of amatoxin groups were much lower than those in the control group. Conclusions The serum levels of hydroxide proline and hexosamine can signal hepatic cell damage induced by amatoxin. They may be the sensitive biochemical indexes in the early clinical diagnosis of amatoxin.