结缔组织生长因子与自发性高血压大鼠心肌纤维化的相关性研究

Study on relation in CTGF and myocardial fibrosis in spontaneously hypertensive rat

目的:探讨结缔组织生长因子(C TG F)在高血压心肌纤维化发生发展中的作用。方法:本实验以14周龄雄性W K Y大鼠和SH R大鼠为研究对象,测量14周、28周大鼠尾动脉压,并对大鼠左室心肌C TG F、TG F-β1的表达用免疫组化法进行半定量分析;R T-PC R法检测C TG F、TG F-β1m R N A表达水平;M A SSO N染色法观察胶原形态,测量胶原容积分数(C V F)和血管周围胶原面积(PV C A)。结果:(1)SH R大鼠组C V F、PV C A明显高于W K Y大鼠组(P<0.01);(2)C TG F、TG F-β1及其m R N A在SH R组左室心肌的表达较W K Y组明显增强(P<0.05)。(3)相关分析表明,C TG F与TG F-β1(r=0.562,P<0.05)、C V F(r=0.715,P<0.01)、PV C A(r=0.786,P<0.01)呈正相关。结论:C TG F参与了SH R大鼠高血压心肌纤维化的病理过程。

Objective To investigate the expression of connective tissue growth factor （CTGF） in the myocardium of spontaneously hypertensive rats and its relationship to the hypertensive myocardial fibrosis, netheds The investigative rats comprised nurmotensive control（WKY） and hypertensive groups（SHR）, of which blood pressure, collagenic content and the expression of CTGF in the left ventricular were measured at 14-and 28-week-old, respectively. CTGF and TGF-β, were evaluated by qualitative and semiquantitative immunohistochemical staining and semiquantitative reverse transcription polymerase chain reaction. MASSON staining was used in the study of total collagen in left ventricular interstitial tissue. Results Compared with the WKY, the collagen volume fraction （CVF） ,perivascular collagen area （PVCA）, and the expression of CTGF, TGF-β1 and the mRNA of them in left ventricular were significantly increased in SHR （P〈0. 05） . CTGF reacted primarily with myocardial interstitium, artery intimal and vascular smooth muscle. Corrective analysis revealed that CTGF positively correlated withTGF-13,（r=0.562, P 〈0.05）, CVF（ r=0. 715, P 〈0.01）and PVCA（r=0.786, P 〈0.01）.Concluslon CTGF may play important roles in developement of myocardial interstistisl fibrosis.