培养幽门螺杆菌-小鼠适应株的实验研究

Study of the H. pylori mouse-passage strains

目的培养幽门螺杆菌-小鼠适应株。方法 (1)先用4株cagA及vacA基因,均为阳性菌株的H．pylori给10只BALB/c小鼠灌胃,4周后剖杀,取小鼠胃黏膜培养及鉴定后得两株(小1、小2) H．pylori-小鼠适应株;(2)用细菌学检查观察小鼠的H．pylori定植情况。结果用4株cagA及vacA基因阳性菌株去感染小鼠,有三只感染成功,感染率为33．3％(3/9),两只传代成功。H．pylori感染小鼠胃黏膜H．pylori定植多位于胃小凹上部及胃腺腔内,数量不多。结论 cagA及vacA基因均为阳性的云南菌株,灌胃后能定植在BALB／c小鼠胃内,培养出H．pylori-小鼠适应株。

Objective 3b isolate H.pylofi mouse-passage strains. Methods （1） H.pylori strains carrying cagA genes and vacA genes were inoculated into stomach of 10 BALMc mice. 4 weeks after the model was made, the stomach biopsies of all the mice were collected to asess the colonization of H. pylori. The H. pylori Mouse-passage strains （litter 1, litter 2） were obtained succesfully. （2） The colonization of H. pylori was assessed by bacterial culture inspection. Results 3 BALMc mice were inoculated orally through a feeding tabe with 4 isolated H. pylori canting cagA genes and vacA genes successfully.The infected rate of H. pylori was 33.3% （3/9）,two strains were transferred successfully. H. pylori was located mainly above gastric pit and less in number. Conclusion The Yunnan H. pylori strains carrying cagA genes and vacA genes can locate in the stomach of BALMc mice after being given orally into mice＇ stomach in which the H. pylori mouse-passage strains can be cultured.