大肠癌组织HLA-I基因蛋白的表达及意义

The protein expression of HLA-I on colorectal carcinoma

目的探讨大肠癌组织HLA-I蛋白的表达。方法 41例大肠癌组织中,14例是高分化腺癌,17例是中分化腺癌,10例是低分化腺癌。用抗HIA-I抗原的单克隆抗体做免疫组织化学分析。结果阳性组织中细胞膜和细胞浆着色。HLA-I在大肠组织中阳性表达率为36．59％,在正常组织中阳性表达率为80％,P<0．05。在不同组织学分级中,HLA-I表达有统计学差异,P<0．05。在淋巴结转移与否的癌组织中HLA-I表达无统计学差异,P>0．05。结论大肠癌组织中HLA-I基因蛋白表达下降, 不同组织学分级表达有统计学差异,淋巴结转移与否无统计学差异。

Objective To measure the protein expression of human leukocyte antigen class I on colorectal carcinoma. Methods Among 41 colorectal carcinoma cases, 14 cases were well differentiated adenocarcinoma, 17 cases were moderate differentiated ones and 10 were low differentiated ones. HLA-I antigens were detected by immunohistochemistry using monoclonal antibody against HLA-I antigens. Results The expression of HLA-I on the positive cell membrane and cytoplasmic expression were observed. The positive rate of HLA-I was 36.59% in colorectal carcinoma.Which was 80% in normal tissues.There was a significant difference between two groups （ P 〈 0.05） .The expression of HLA-I in cancer tissues was correlated statistically with the degree of differentiation （ P 〈 0.05） .The expression in cases with lymph node metastasis was not correlated statistically with those without lymph node metastasis （ P 〉 0.05）. Conclusion The expression of HLA-I is lower in colorectal carcinoma than that in normal tissues and positively correlated with histological grade of cancer, but not with lymph node metastasis.