细胞外基质蛋白-1与喉癌血管生成和转移的关系

The relationship between ECM1 and the angiogenesis and metastasis of laryngeal carcinoma

目的:研究细胞外基质蛋白1(ECM1)与喉癌生长、转移和血管生成的关系。方法:用免疫组织化学方法检测33例喉良恶性肿瘤ECM1和Ⅷ因子相关抗原的表达情况。用RT-PCR分子技术检测5例新鲜喉癌组织及其癌旁组织的ECM1mRNA的表达情况。结果:喉良、恶性两组肿瘤组织ECM1的表达比较差异有统计学意义(P<0.01)。喉癌组ECM1表达明显强于良性组。喉癌组ECM1表达与喉癌微血管密度(24.50±5.96)存在正相关(γ=0.505,P<0.05)。5例喉癌组织及其癌旁组织均检测到ECM1mRNA的表达,两者mRNA的表达量差异不明显。结论:ECM1在喉癌发生、发展过程中起促进癌细胞生长、转移和具有血管生成作用。ECM1的深入研究有利于治疗喉癌和判断喉癌预后。

Objective：To investigate the expressions of extracellular matrix protein-1（ECM1）in laryngeal carcinoma and their relationships with the growth, metastasis and angiogenesis of tumor. Method： The immunohistchemical technique was used to detect the expressions of ECM1 and Ⅶ factor in 33 cases of laryngeal tumors . RT-PCR technology was used to test the existence of ECM1 mRNA in both fresh laryngeal tissue and its surrounding tissue （total 5 cases）. Result： ECM1 is expressed in both benign and malignant laryngeal tissues, chiefly in the cellular plasma. But the expression of ECM1 （ P〈0.01） is significant different between benign and malignant laryngeal tumors. Their expressions are stronger in malignant tissues than in benign ones. In malignant laryngeal tissue, the expression of ECM1 is associated significantly with microvessel density （MVD）（24.50 ± 5.96） （Spearman＇s rho is 0. 505, P〈0.05）. ECM1 mRNA is found in both laryngeal carcinoma tissue and its surrounding tissue and it seemed no difference. Conclusion： ECM1 played an important role in the growth, metastasis and angiogenesis of laryngeal carcinoma. The further investigation of ECM1 will benefit the treatment of laryngeal carcinoma and the judgement of its prognosis.