子宫颈鳞癌HPV16感染对错配修复基因表达的影响

Effect of human papillomavirus 16 infection on expression of mismatch repair gene in cervical squamous cancer

目的:探讨子宫颈癌发病机制中HPV感染对错配修复(mismatchrepair,MMR)基因表达影响的意义。方法:77例子宫颈鳞癌标本采用免疫组织化学SP法检测HPV16、hMSH2与hMLH1的表达。并分析这些表达与临床病理参数的关系。结果:HPV感染与非感染组子宫颈癌hMLH1的阳性表达率分别为69.0%(29/42)和40.8%(12/28),rs=0.260,P=0.029;hMSH2的阳性表达率分别为64.2%(27/42)和46.4%(13/28),rs=0.177,P=0.143。HPV感染、hMLH1及hMSH2阳性表达率与分化程度、临床分期及淋巴结转移无显著相关。结论:HPV感染组细胞hMSH2与hMLH1蛋白表达上调的现象表明HPV16感染增加子宫颈上皮细胞DNA复制时的碱基错配。因此,这可能是HPV16的致癌机制之一。

OBJECTIVE.. To study the significance of the effect of Human Papillomavirus （HPV） 16 infection on expression of Mismatch Repair Gene in the mechanism of cervical squamous cancer. METHODS： Paraffin specimens of 77 cervical squamous cancer from the Obstetrics and Gynecology Hospital of Dalian was used to test the protein expressions of HPV16, hMLH1, hMSH2 with the methods of immunohistochemistry staining （SP）. The relationship of each expressions and the correlation between the expressions and clinical pathological features were analyzed. RESULTS： The expression rates of hMLH1 were 69.0 % （29/42） and 40.8% （12/28） in the HPV16 infection group and no infection group, respectively, being significant higher in the infection group, r,=0. 260,P〈 0.05; expression rates of hMSH2 were 64.2M （27/42） and 46.4%（13/28）, respectively, in the HPV16 infection group and no infection group, being not significant higher in the infection group, r, = 0. 177,P〉 0.05. There were no significant differences between positive protein expression of HPV16, hMLH1 and hMSH2 and clinical pathological features including differentiation grades, FIGO stages and lymph node transferences. CONCLUSIONS： The increased positive protein expressions of hMSH2 and hMLH1 in the group of HPV16 infection indicate that HPV16 can increase cervical epithelial cells mismatched bases at the time of DNA replication. So, it is likely one of the mechanisms of HPV16 to lead to cervical cancer.