云南松成熟胚的离体培养

In Vitro Culture of Mature Embryos of Pinus yunnanensis

采用云南松(yunnanensis)成熟胚为外植体,培养室温度为25±4℃,培养基的蔗糖浓度为8％。以 1／2MS为基本培养基,在BA0．5-2．0mg／L+NAA0．5-1．5mg／L的激素配比范围内,可诱导离体胚产生愈伤组织及不定芽。外植体愈伤组织的诱导率达80％,由愈伤组织诱导不定芽,其分化率达17．8％。研究结果表明,激素的种类和浓度对外植体愈伤组织及不定芽的诱导起主要作用。

Explants were initiated from mature embryos of pinus yunnanensis, All the cultures were kept in culture room at 24±4℃. The medium contained8%sucrouse. Callus and ad ventitious bud were induced from excised embryos onl/2MS medium supplemented with BA0. 5- 2. 0mg/L and NAA0.5-1.5mg/L. Theinduction rate of calli from plants was 80%, The differentiation rate of adventitious buds from callus was 17.8%. The results showed that the various concentrations and combinations of hormones played a determinative role in inducing Callus from plants and adventitious buds from callus.