摘要
objective: construct a homologous recombinant plasmid which was expected to be transformed into S.mutans Methods: a region at the 5’terminus of the S.mutans F-ATPase β subunit gene was amplified by PCR, the PCR product was inserted into vector pVA891,yielding recombinant plasmid.Results: the DNA sequence of the recombinant plasmid was identified correct in whole by restriction endonuclease and DNA sequence techniques.Conclusion: the recombinant plasmid of S.mutans DNA was cloned in effect,it may assist in construction of homologues recombinant mutant.
objective： construct a homologous recombinant plasmid which was expected to be transformed into S. mutans Methods ： a region at the 5＇ terminus of the S. mutans F-ATPase β subunit gene was amplified by PCR, the PCR product was inserted into vector pVA891, yielding recombinant plasmid. Results： the DNA sequence of the recombinant plasmid was identified correct in whole by restriction endonuclease and DNA sequence techniques. Conclusion： the recombinant plasmid of S. mutans DNA was cloned in effect,it may assist in construction of homologues recombinant mutant.
