高原紫外线对人体表皮郎格罕细胞影响的免疫组化研究

AN IMMUNOHISTOCHEMICAL STUDY OF EPIDERMIS LANGERHAN'S CELLS IN HUMAN EXPOSURED TO ULTRAVIOLET LIGHT AT HIGH ALTITUDE

青海格尔木地区紫外线的年平均强度是青岛等沿海地区夏季中午时分的１．７２倍，高强度的紫外线对高原居民皮肤造成很大影响。我们取这一地区生活的农牧民、地质工作者中志愿者手背部（暴露组）及肩关节凸面（非暴露组）皮肤组织。用ｌｅｕ６单克隆抗体及Ｓ－１００蛋白多克隆抗体免疫组化方法对比研究在皮内ｌａｎｇｅｒｈａｎｓ细胞损伤情况。结果：①暴露组表皮内ｌｅｕ６阳性细胞明显少于非暴露组（Ｐ＜０．０１）；②在表皮内郎格罕化细胞的标记，ｌｅｕ６单克隆抗体显示优于Ｓ－１００蛋白。

he intensity of ultraviolet light in Geermu(2 800m altitude)(0.186J/cm/min)was 1.72times of sea level.To sttidy the effects of tiltraviolet light(UL)on skin at altitude,the number oflangerhan's cells of epidermis were cotinted at UL-expostired skin site(back of left hand)and UL-unexpostired site(exteiiLsion side of shpuledr joint)in the same subjects,with Leu6 and S-100 pro-tein immunohistochemical stain.The results showed that the number of langerhan's cells in expo-sured sites reduced markedlly as compared with unexposurecl sites(P<0.01).Most of the S-100protein positive cells were located in the basal layer of epiclermis.It was concluded that the ultra-violet light was harmful to the epidermis langerhan's cells,and in labeling langerhan's cells andindemonstrating the cell's protrusion the Leu6 was more specific and better than S-100 protein.