抗人肝癌细胞单克隆抗体免疫组化及免疫电镜研究

A STUDY ON IMMUNOHISTOCEMIStRY AND IMMUNO-ELECTRON-MICROSCOPY WITH A MONOCLONAL ANTIBODY TO HUMANHEPATOCELLULAR CARCINOMA

本文用抗人肝癌细胞的单克隆抗体,采用ABC法,50例临床病理诊断为肝细胞肝癌及正常组织等的石蜡切片进行了免疫组化研究,发现该抗体染色在肝癌阳性率为88％(44/50例),而在全身绝大部份脏器的正常组织无交叉反应。这一结果表明,该抗体对肝癌组织具有一定的特异性。为证实此抗体定位的相应抗原部位,我们还通过免疫电镜包埋前和包埋后两种不同的染色方法,对5例临床手术切除的新鲜肝癌标本进行了超微结构定位观察。结果,此抗体主要定位于细胞膜性结构,包括细胞膜连接,线粒体膜和内质网膜。推测此抗体为肝癌细胞相关膜抗原的单克隆抗体。此抗原物质的发现,为肝癌诊断及研究展示了新的前景。

Using monoclonal antibody (McAb) to human hepatoma combined withthe avidin-biotin-peroxidase complex (ABC) immunostaining method, the immunohistochemistry of the paraffin sections 50 cases of- hepatoma and normal tissues was studied. The rusults showed that 44 cases of hepatoma were stained positively, the rate of positivity was 88% Most normal tissues, were stained negatively. This indicated that the antibody is specific to human hepato-cellular carcinoma. Both pre-and post-embedding immunoelectron-microscopy were used to define the ultrastnictural distribution of this antigen. The results demonstrated that the monclonal antibody was localized in membranous structures of the cell (plasma membrane; mitochondrial and endoplasmic reticulum membrane). We suggested that the monoclonal antibody is associated with the membrane antigen of human hepatoma.