利用HPLC和GC全面分析葡萄醋中有机酸

Determination of carboxylic acids in grape vinegars by HPLC and GC methods

葡萄醋成分复杂,对有机酸的准确测定影响很大,本实验采用两步固相萃取技术除杂,比较了外标法和标准加入法定量的优劣,分析了HPLC和GC方法各自的局限性。

The determination of organic acids in grape vtnegars is often diffieult because of the presence of numerous interferences that need to be removed by separation techniques. In this experiment, a solid-phase extraction （SPE） method with C18 and NH2 exchangers was used to clean the samples prior to analysis or further treatments. Both HPLC and GC techniques were used to determine organic acids. The efficiency of these two different analytical techniques in the study of grape vinegars acidic composition has been evaluated.