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拟南芥T-DNA插入突变体ATSUC3的PCR鉴定 被引量:28

Identification of atsuc3 with T-DNA Insertion by PCR
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摘要 应用两种植物T-DNA插入突变体PCR鉴定方法,即“三引物法”和“双引物法”对拟南芥T-DNA插入突变体ATSUC3(目的基因两条染色体均发生T-DNA插入的纯合突变体)进行鉴定和比较的结果表明,“三引物法”由于易产生非特异性扩增而无法得到PCR鉴定结果,从而导致鉴定失败;“双引物法”则可避免此种现象,得到可靠、有效的鉴定结果。
出处 《植物生理学通讯》 CSCD 北大核心 2006年第1期91-94,共4页 Plant Physiology Communications
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参考文献8

  • 1杨双,阮燕晔,樊金娟,张立军.一种简易的拟南芥幼苗微量DNA提取方法[J].沈阳农业大学学报,2005,36(1):99-100. 被引量:21
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二级参考文献4

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