摘要
本文对9例口腔癌手术切除的原发灶肿瘤标本的TIL进行了体外分离、培养、扩增、表型分析及NK和LAK活性测定。其中8例扩增获得成功,扩增倍数为10~1200(平均472.2)倍。表型分析表明,扩增后的TIL中CD,CD和CD均有增加,但以CD、CD为主。抗瘤活性在培养4周左右达最高峰;NK和LAK活性分别为52%和44%。全部病例经肉汤培养基、沙氏培养基、支原体检测药盒及家兔发热试验未检测到细菌、真菌、支原体及热原质。本研究提示,口腔癌TIL的临床应用是可行的。
umor Infiltrating Lymphocytes(TILs)isolated ftom 9 patients with oral cancer were cultured with low concentration of YIL-2 in vitro.The phenotypes were ana lysed by the method of immunocytochemistry,and the anti-tumor activities were determined by 3H-TDR release assay.The results showed that TILs from 8 of 9 cases,were able to be expanded 100-1200 folds,the median was 472.2,and expressed significant cytotoxicity against both NK-Seneitive K562 and LAK-Sens itive Raji targets.When activited and expanded with YIL-2 for four weeks,TILs expressed and predominantly,though increased much.Culture with both bouillon Sabouraud's medium and GiBco Mycotect kit showed no contaDination of bacteria,fungi and nycoplasm in the rIL-2 cxpanded TILs.and pyrogenic test excluded the contamination of pyrogen.This study denonstrates that TILs from patients with oral cancer can be used possibly and safely in clinic.
出处
《口腔颌面外科杂志》
CAS
1996年第2期95-98,共4页
Journal of Oral and Maxillofacial Surgery
基金
卫生部科研基金
关键词
肿瘤浸润
淋巴细胞
口腔肿瘤
制备
Tumor infiltrating lymphocytes(TILs)Oral cancer Microorganism