阿霉素和生长因子对FUKU细胞的生长和克隆形成的作用研究

THE EFFECTS OF ADRIAMYCIN AND GROWTH FACTORS ON CELL GROWTH AND COLONY FORMING EFFICIENCY OF FUKU CELLS

本文应用不同浓度的阿霉素,观察其对小鼠乳腺癌的表皮细胞株—FUKU细胞的生长和克隆形成的影响,研究结果表明,阿霉素浓度为0.1—1.0ng/ml时,其对FUKU细胞生长抑制作用不甚明显;阿霉素浓度为10ng/ml时.其对FUKU细胞生长抑制率为正常对照的50％左右;当阿霉素浓度为60—1500ng/ml时,FUKU细胞的生长几乎完全受抑制。阿霉素对FUKU细胞的克隆形成的抑制作用较其对FUKU细胞生长抑制作用更为显著,FUKU细胞的克隆形成的抑制不仅与阿霉素浓度相关,更重要与作用时间有关。当作用时间为72—120小时时,小剂量阿霉素(0.01ng/ml)也可有效抑制FUKU细胞的克隆形成。此外,FUKU细胞克隆形成能力恢复不仅决定于阿霉素的浓度,还取决于阿霉素的作用时间。转化生长因子和表皮生长因子对经阿霉素作用后的FUKU细胞的克隆形成并无明显刺激作用。

The effect of adriamycin on cell growth and colony forming efficiencyof FUKU cells, a murine mammary tumor epithelial cell line, was studied. The results revealed that there was no significant difference between the cell number treated with adriamycin at the concentration of (0.1-1.0ng/ml) and recovered in the control. When the concentration of adriamycin was 10ng/ml, the inhibition of cell growth was 50% of normal control, the inhibition of cell growth was almost completely under the concentration of adriamycin of 60-1500ng/ml. The effect of adriamycin on colony forming efficiency was more significant than that on cell growth. The inhibition of adriamycin on colony forming efficiency was not only dependent on the concentration, but also the treatment duration of adriamyiin. The recovery of colony forming efficiency of FUKU cell treated with adriamycin was also dependent on the concentration and the treatment duration of adriamycin, TGF-α ,TGF-β, and EGF could not stimulate the colony forming efficiency of FUKU cells treated by adriamycin.