PVP包覆β-榄香烯脂质体的处方与工艺优化

Optimal preparation of PVP-complexedβ-elemene liposomes

目的:考察3种不同相对分子质量的聚乙烯毗咯烷酮(PVP)包覆的β-榄香烯脂质体的包封率,确定最终处方。方法:采用正交设计优化处方与制备工艺;采用乙醇注入法制备不同相对分子质量PVP包覆的β-揽香烯脂质体;采用微柱离心-气相色谱法测定脂质体包封率。结果:正交试验结果证明利用1%PVP-k30包覆,载药量为0．5%,磷脂用量为6%,磷脂与胆固醇质量比例为6:1为最佳处方。结论:脂质体用PVP包覆后包封率为(97．19±0．27)%,与普通脂质体相比未见明显变化,粒径则略有增加。

Objective： To assess the inclusion rate of β-elemene liposomes complexes with 3 kinds of polyvinylpyrrolidones （PVPs）. Methods：The formulation and preparation were optimized by an orthogonal test. The liposomes inclusion complexes were made using an ethanol injection method, The inclusion rate was measured by a minicolumn centrifugation-gas chromatography method. Results：The optimal formulation was composed of 1% PVP-k30, 0.5%β-elemene, 6% phospholipids and 1% cholesterol. The inclusion rate was （97.19 ±0.27）% , which showed no significant difference from conventional liposomes except a slight increase of particle diameter. Conclusion：The PVP-complexed β-elemene liposomes were attained.