RP-HPLC法测定血浆中阿魏酸哌嗪浓度

Determination of piperazine feralate in human plasma by RP-HPLC

目的:建立血浆中阿魏酸哌嗪浓度的反相高效液相色谱法。方法:血浆标本以西沙比利为内标,经酸化和二氯甲烷提取后,以0．02 mol·L^(-1)磷酸二氢钠(pH2．7)-甲醇(45:55)为流动相,经碳十八烷基键合硅胶柱分离,在紫外310nm处检测。结果:阿魏酸哌嗪标准曲线范围0．016～16 mg·L～(-1),(r=0．9999),最低定量限为0．016 mg·L～(-1),高、中、低4种浓度的日内精密度均＜4%,日间精密度均＜6%,方法回收率93．8%～102．3%。结论:本法简单可靠,可用于临床阿魏酸哌嗪的药动学和药效学研究。

Objective：To set up a RP-HPLC method to determine the concentration of piperazine feralate in human plasma. Methods：Cisapride was used as an internal standard. The plasma samples acidly pre-treated with 4 mL CH2Cl2 were assayed over a C18 （2） （PHENOMENEX Luna） （250 mm×4.6 mm,5μm, NO. 74434-13 ） and an eluate consisted of CH3 OH： 0.02 mol·L^-1 Nah2PO4 （ pH2.7, adjust with H3PO4） （55：45 ） with a detection at 310 nm. Results：The calibrated linear curve of piperazine feralate was in the range of 0. 016 ～ 16.0 mg·L^ - 1 （ r = 0. 999 9）. The detection limit was 0. 016 mg·L^ - 1 and the recovery rate was 93.8% ～ 102.3%. The within-day and among-day RSD was less than 4% and less than 6% , respectively. Conclusion：The simple, rapid and sensitive RP-HPLC could be used in the investigations of pharmacokinetics and relative bioavailability of piperazine feralate.