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野生型K-RAS2基因对人结肠癌细胞生长的抑制作用 被引量：2

Growth inhibitary effect of wild-type K-ras2 gene on colon adenocarcinoma cell
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摘要 目的:研究野生型K-ras2基因对人结肠癌细胞株Caco-2的生长影响作用。方法:将野生型K-ras2基因转染结肠癌细胞株Caco-2并筛选稳定表达的克隆;用四甲基偶氮唑(MTT)法观察细胞的生长情况,用流式细胞仪观察细胞周期及凋亡的情况。结果:野生型K-ras2基因可使结肠癌细胞株Caco-2的生长明显受到抑制,使细胞受阻于G0/G1期。结论:野生型K-ras2基因能有效抑制结肠癌细胞Caco-2的生长。 Objective：To observe the growth inhibitary effect of wild-type K-ras2 gene on colon adenocarcinoma cell line Caco-2. Methods：The colon adenocarcinoma cell line Caco-2 was transfected with the wild-type K-ras2 gene, and stable expression clones were selected. The growth of the colonadenocarcinoma cell was detected by monotetrazolium（MTr） , and the cell cycle analysis was carried out by flow cytometry （FCM）. Results：The expression of wild-type K-ras2 gene observably suppressed the growth of the colon adenocarcinoma cell line Caco-2 and arrested cell cycle in the G0/G1 phase. Conclusion：The wild-type K-ras2 gene effectively suppressed the growth of colon adenocarcinoma cell line Caco-2.
作者 朱美玲 万军 李红 李园
机构地区 解放军总医院南五科
出处 《军医进修学院学报》 CAS 北大核心 2006年第1期18-19,共2页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金资助项目(30200326)
关键词 K-ras2 结肠肿瘤 细胞周期 K-ras2 colonic neoplasms cell cycle
分类号 R574 [医药卫生—消化系统]
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参考文献9

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共引文献18

同被引文献33

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引证文献2

军医进修学院学报
2006年 第1期

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