慢性坐骨神经损伤大鼠海马GFAP和pERK表达的变化

Expression of glia fibrillary acidic protein and phosphorylated extracellular signal-regulated kinase in the hippocampus of chronic constriction injury rats

目的:探讨慢性坐骨神经挤压损伤(CC I)大鼠海马组织中胶质原纤维酸性蛋白(GFAP)和磷酸化有丝分裂素激活蛋白激酶(pERK)表达的改变。方法:按Bennett法制作CC I模型,采用免疫组化法测定CC I大鼠海马组织中GFAP和pERK的表达。结果:CC I大鼠双侧海马齿状回胶质细胞GFAP和pERK表达均有增加,以手术对侧增加更为显著,与假手术组比较差异有显著性(P<0.01)。结论:中枢神经系统胶质细胞ERK/MAP激酶通路激活,可能参与慢性神经痛的发病过程。

Objective：To determine the expression of glia fibrillary acidic protein（GFAP） and phosphorylated extraeellular signal-regulated kinase（pERK） in the hippocampus of chronic constriction injury（CCI） rats. Methods：The rats were placed four loose ligatures around the right sciatic nerve and the changes of GFAP and pERK protein expression in the hippocampus was detected by immuno-histochemieal techniques. Results： Immunoreactivity （IR） of GFAP, pERK protein was significantly increased in the contralateral hippocampus in CCI group（P 〈 0.01 ）. Conclusion： CCI may activate ERK/MAP （Mitogen activated protein） kinase pathways in astrocytes in the hippocampus, and ERK/MAPK pathways may play an important role in the genesis of neuropathic pain.